In vitro and in silico inhibition of Staphylococcus aureus efflux pump NorA by α‑Pinene and Limonene

dc.creatorAraújo, Ana Carolina Justino de
dc.creatorFreitas, Priscilla Ramos
dc.creatorBarbosa, Cristina Rodrigues dos Santos
dc.creatorMuniz, Débora Feitosa
dc.creatorAlmeida, Ray Silva de
dc.creatorMenezes, Irwin Rose Alencar de
dc.creatorRibeiro Filho, Jaime
dc.creatorTintino, Saulo Relison
dc.creatorCoutinho, Henrique Douglas Melo
dc.date2021-08-31T11:35:43Z
dc.date2021-08-31T11:35:43Z
dc.date2021
dc.date.accessioned2023-09-26T20:58:09Z
dc.date.available2023-09-26T20:58:09Z
dc.identifierARAÚJO, Ana Carolina Justino de et al. In vitro and in silico inhibition of Staphylococcus aureus efflux pump NorA by α‑Pinene and Limonene. Current Microbiology, v. 78, p. 3388-3393, 16 July 2021.
dc.identifier0343-8651
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/48850
dc.identifier10.1007/s00284-021-02611-9
dc.identifier1432-0991
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867002
dc.descriptionSince the discovery of the first antibiotics, bacteria have acquired a variety of resistance mechanisms, with efflux pump (EP) being the most prominent mechanism for intracellular targeting drugs. These proteins have become efficient mechanisms of resistance to antibiotics in species such as Staphylococcus aureus and, therefore, have been identified as promising therapeutic targets in antibacterial drug development. Accordingly, evidence suggests that monoterpenes can act as EP inhibitors and can be useful in circumventing bacterial resistance. This study aimed to evaluate the effectiveness of monoterpenes α-pinene and limonene as EP inhibitors against a strain of S. aureus expressing NorA protein. The minimum inhibitory concentration (MIC) against the 1199B strain of S. aureus, which carries genes encoding efflux proteins associated with antibiotic resistance to norfloxacin, was assessed through the broth microdilution method. The results obtained served as a subsidy for the analysis of the NorA pump inhibition with norfloxacin and ethidium bromide. Docking techniques, in silico, were used to evaluate the interaction of monoterpenes with NorA. Both monoterpenes showed no clinically effective antibacterial activity. Nevertheless, these compounds were found to decrease the MICs of ethidium bromide and norfloxacin indicating EP inhibition, which was confirmed by molecular docking analyses. In conclusion, α-pinene and limonene showed promising antibiotic-enhancing properties in S. aureus 1199B strain, indicating that monoterpenes can be used in targeted drug development to combat antibiotic resistance associated with EP expression.
dc.description2022-07-16
dc.formatapplication/pdf
dc.formatapplication/vnd.openxmlformats-officedocument.wordprocessingml.document
dc.languageeng
dc.publisherSpringer
dc.rightsrestricted access
dc.subjectStaphylococcus aureus
dc.subjectLimoneno
dc.subjectResistência Microbiana a Medicamentos
dc.subjectTécnicas In Vitro
dc.subjectStaphylococcus aureus
dc.subjectDrug Resistance, Microbial
dc.subjectIn Vitro Techniques
dc.subjectLimonene
dc.titleIn vitro and in silico inhibition of Staphylococcus aureus efflux pump NorA by α‑Pinene and Limonene
dc.typeArticle


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