Microanatomy of the American Malaria Vector Anopheles aquasalis (Diptera: Culicidae: Anophelinae) Midgut: Ultrastructural and Histochemical Observations

dc.creatorSilva, Djane Claris Baia da
dc.creatorOrfanó, Alessandra da Silva
dc.creatorPimenta, Rafael Nacif
dc.creatorMelo, Fabricio Freire de
dc.creatorVale, Maria das Graças
dc.creatorLacerda, Marcus Vinícius Guimarães de
dc.creatorMonteiro, Wuelton Marcelo
dc.creatorPimenta, Paulo Filemon Paolucci
dc.date2020-02-17T18:09:40Z
dc.date2020-02-17T18:09:40Z
dc.date2019
dc.date.accessioned2023-09-26T20:57:54Z
dc.date.available2023-09-26T20:57:54Z
dc.identifierSILVA, Djane Claris Baia da et al. Microanatomy of the American Malaria Vector Anopheles aquasalis (Diptera: Culicidae: Anophelinae) Midgut: Ultrastructural and Histochemical Observations. Journal of Medical Entomology, v. 56, n. 6, p. 1636-1649, 2019.
dc.identifier0022-2585
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40015
dc.identifier10.1093/jme/tjz114
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866936
dc.descriptionThe mosquito gut is divided into foregut, midgut, and hindgut. The midgut functions in storage and digestion of the bloodmeal. This study used light, scanning (SEM), and transmission (TEM) electron microscopy to analyze in detail the microanatomy and morphology of the midgut of nonblood-fed Anopheles aquasalis females. The midgut epithelium is a monolayer of columnar epithelial cells that is composed of two populations: microvillar epithelial cells and basal cells. The microvillar epithelial cells can be further subdivided into light and dark cells, based on their affinities to toluidine blue and their electron density. FITC-labeling of the anterior midgut and posterior midgut with lectins resulted in different fluorescence intensities, indicating differences in carbohydrate residues. SEM revealed a complex muscle network composed of circular and longitudinal fibers that surround the entire midgut. In summary, the use of a diverse set of morphological methods revealed the general microanatomy of the midgut and associated tissues of An. aquasalis, which is a major vector of Plasmodium spp. (Haemosporida: Plasmodiidae) in America.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.rightsrestricted access
dc.subjectAnopheles aquasalis
dc.subjectFluorescent lectin labeling
dc.subjectMidgut
dc.subjectUltrastructure
dc.titleMicroanatomy of the American Malaria Vector Anopheles aquasalis (Diptera: Culicidae: Anophelinae) Midgut: Ultrastructural and Histochemical Observations
dc.typeArticle


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