Differential gene expression in oyster exposed to sewage

dc.creatorMedeiros, Igor Dias
dc.creatorSiebert, Marília Nardelli
dc.creatorSilva, Guilherme de Toledo e
dc.creatorMoraes, Milton Ozório
dc.creatorMarques, Maria Risoleta Freire
dc.creatorBainy, Afonso Celso Dias
dc.date2019-03-07T14:17:46Z
dc.date2019-03-07T14:17:46Z
dc.date2008
dc.date.accessioned2023-09-26T20:57:36Z
dc.date.available2023-09-26T20:57:36Z
dc.identifierMEDEIROS, Igor Dias; et al. Differential gene expression in oyster exposed to sewage. Marine Environmental Research, v.66, p.156–157, 2008.
dc.identifier0141-1136
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31970
dc.identifier10.1016/j.marenvres.2008.02.048
dc.identifier1879-0291
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866849
dc.descriptionIn order to investigate the influence of domestic sewage on the gene expression of Pacific oysters Crassostrea gigas, suppression subtractive hybridization (SSH) method was employed. Oysters were sampled at a farming area and, after 10 days of acclimation in the laboratory, were exposed to untreated domestic sewage diluted 33% for 48 h. Gills of male oysters were excised for total RNA extraction. mRNA was purified and the differential gene expression was analyzed by SSH. We obtained 61 cDNA sequences but only 15 were identified, which includes fatty acid binding protein, multidrug resistance protein, omega glutathione S-transferase, cytochrome P450 isoform CYP356A1, among others. The identified genes are associated with different metabolic functions like biotransformation, membrane transport, aerobic metabolism and translational machinery, evidencing the potential toxic effects elicited by these effluents.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectCrassostrea gigas
dc.subjectEsgoto
dc.subjectExpressão genetica
dc.subjectMonitoramento
dc.subjectBiomarcador
dc.subjectCrassostrea gigas
dc.subjectSSH
dc.subjectGene expression
dc.subjectSewage
dc.subjectBiomarker
dc.subjectMonitoring
dc.titleDifferential gene expression in oyster exposed to sewage
dc.typeArticle


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