Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells

dc.creatorAnsa-Addo, Ephraim A.
dc.creatorLange, Sigrun
dc.creatorStratton, Dan
dc.creatorAntwi-Baffour, Samuel
dc.creatorCestari, Igor
dc.creatorRamirez, Marcel I.
dc.creatorMcCrossan, Maria V.
dc.creatorInal, Jameel M.
dc.date2017-09-12T18:07:27Z
dc.date2017-09-12T18:07:27Z
dc.date2010
dc.date.accessioned2023-09-26T20:56:52Z
dc.date.available2023-09-26T20:56:52Z
dc.identifierANSA-ADDO, Ephraim A. et al. Human Plasma Membrane-Derived Vesicles Halt Proliferation and Induce Differentiation of THP-1 Acute Monocytic Leukemia Cells. The Journal of Immunology, v.185, p.5236-5246, Oct. 2010.
dc.identifier0022-1767
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20984
dc.identifier10.4049/jimmunol.1001656
dc.identifier1550-6606
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866641
dc.descriptionPlasma membrane-derived vesicles (PMVs) are small intact vesicles released from the cell surface that play a role in intercellular communication. We have examined the role of PMVs in the terminal differentiation of monocytes. The myeloid-differentiating agents all-trans retinoic acid/PMA and histamine, the inflammatory mediator that inhibits promonocyte proliferation, induced an intracellular Ca(2+)-mediated PMV (as opposed to exosome) release from THP-1 promonocytes. These PMVs cause THP-1 cells to enter G(0)-G(1) cell cycle arrest and induce terminal monocyte-to-macrophage differentiation. Use of the TGF-β receptor antagonist SB-431542 and anti-TGF-β1 Ab showed that this was due to TGF-β1 carried on PMVs. Although TGF-β1 levels have been shown to increase in cell culture supernatants during macrophage differentiation and dendritic cell maturation, the presence of TGF-β1 in PMVs is yet to be reported. In this study, to our knowledge we show for the first time that TGF-β1 is carried on the surface of PMVs, and we confirm the presence within PMVs of certain leaderless proteins, with reported roles in myeloid cell differentiation. Our in vitro findings support a model in which TGF-β1-bearing PMVs, released from promonocytic leukemia cells (THP-1) or primary peripheral blood monocytes on exposure to sublytic complement or after treatment with a differentiation therapy agent, such as all-trans retinoic acid, significantly reduce proliferation of THP-1 cells. Such PMVs also induce the terminal differentiation of primary peripheral blood monocytes as well as THP-1 monocytes.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Association of Immunologists
dc.rightsrestricted access
dc.subjectVesículas plasmáticas derivadas da membrana
dc.subjectPerda
dc.subjectLeucemia Monocítica Aguda
dc.subjectInduzem a diferenciação de THP-1
dc.subjectPlasma membrane-derived vesicles
dc.subjectAcute Monocytic Leukemia
dc.subjectInduce Differentiation of THP-1
dc.titleHuman plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells
dc.typeArticle


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