Use of sorghum seed tissue as a biocatalyst in a stirred reactor for oxalic acid determination

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFernandes, João Roberto
dc.creatorDe Oliveira Neto, Graciliano
dc.creatorKubota, Lauro T.
dc.creatorTubino, Matthieu
dc.date2014-05-27T11:18:07Z
dc.date2016-10-25T18:14:01Z
dc.date2014-05-27T11:18:07Z
dc.date2016-10-25T18:14:01Z
dc.date1996-11-01
dc.date.accessioned2017-04-06T00:47:56Z
dc.date.available2017-04-06T00:47:56Z
dc.identifierAnalytical Communications, v. 33, n. 11, p. 397-399, 1996.
dc.identifier1359-7337
dc.identifierhttp://hdl.handle.net/11449/64871
dc.identifierhttp://acervodigital.unesp.br/handle/11449/64871
dc.identifier10.1039/AC9963300397
dc.identifierWOS:A1996VT08900003
dc.identifier2-s2.0-0000678174
dc.identifierhttp://dx.doi.org/10.1039/AC9963300397
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/886648
dc.descriptionThe enzyme oxalate oxidase, E.C. 1.2.3.4 from Sorghum vulgare seeds (variety BR303) was used to develop a new sensor for oxalate determination without any purification. The sorghum seeds were conditioned in a 0.10 mol I-1 KCl solution. Then, these seeds were put in a stirring bar type enzymic reactor and coupled with an electrode for CO2. This device was introduced into a cell containing 10.0 ml of a 0.10 mol I-1 KCl solution saturated with oxygen. This sensor showed a linear response between 1.0 and 4.0 × 10-3 mol I-1 with a slope of 30 mV per decade of oxalate concentration at 25.0°C. The sensor was stable for one month or 200 determinations. The response time was about 60 s. The Michaelis-Menten constant determined for this enzyme was 1.5 × 10-3 mol I-1.
dc.languageeng
dc.relationAnalytical Communications
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleUse of sorghum seed tissue as a biocatalyst in a stirred reactor for oxalic acid determination
dc.typeOtro


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