Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase

dc.creatorReis, Mitermayer Galvão dos
dc.creatorDavis, Richard E
dc.creatorSingh, Hardeep
dc.creatorSkelly, Patrick J
dc.creatorShoemaker, Charles B
dc.date2014-05-05T19:24:49Z
dc.date2014-05-05T19:24:49Z
dc.date1993
dc.date.accessioned2023-09-26T20:55:24Z
dc.date.available2023-09-26T20:55:24Z
dc.identifierREIS, M. G dos et al. Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase. Molecular Biochemical Parasitology, v. 59, n. 2, p. 235-242, 1993.
dc.identifier0166-6851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7580
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866221
dc.descriptionThe complete gene encoding Schistosoma mansoni triosephosphate isomerase (TPI) was isolated from a lambda phage genomic library on 2 overlapping clones. These genomic clones have been characterized by restriction mapping and DNA sequencing of the 5' flanking region, the exons, the intron boundaries and the polyadenylation addition site. S. mansoni TPI is encoded by 6 exons spanning a region of about 12 kb. The 5 introns are located at positions precisely analogous to those of mammalian TPI genes but one of the 6 mammalian TPI introns is missing in S. mansoni. We find no evidence of spliced leader involvement in TPI gene expression. The gene is preceded by at least 4 tandem copies of a 2.5-kb repetitive sequence. While the 12-kb size for the S. mansoni TPI gene is much larger than the 3-4 kb typical of mammalian TPI genes, the 42-bp first intron is unusually short. The transcription initiation site for the S. mansoni TPI gene is heterogeneous. Genomic Southern blot analysis suggests that TPI is expressed from a single copy gene.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Science Publishers B.V
dc.rightsopen access
dc.subjectSchistosome
dc.subjectSchistosoma mansoni
dc.subjectTPI
dc.subjectGlycolysis
dc.subjectIntron
dc.subjectGene
dc.subjectAnimais
dc.subjectSchistosoma mansoni/enzimologia
dc.subjectSchistosoma mansoni/genética
dc.subjectTriose-Fosfato Isomerase/genética
dc.subjectSequência de Aminoácidos
dc.subjectNorthern Blotting
dc.subjectClonagem Molecular
dc.subjectDNA/análise
dc.subjectDNA/isolamento & purificação
dc.subjectSequência de Bases
dc.subjectBiblioteca Genômica
dc.subjectGlicólise
dc.subjectDados de Sequência Molecular
dc.subjectOligodesoxirribonucleotídeos
dc.subjectReação em Cadeia da Polimerase
dc.subjectRNA Mensageiro/isolamento & purificação
dc.titleCharacterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase
dc.typeArticle


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