Isolation and characterization of trypanosomatids, including Crithidia mellificae, in bats from the Atlantic Forest of Rio de Janeiro, Brazil

dc.creatorRangel, Diana Azevedo
dc.creatorLisboa, Cristiane Varella
dc.creatorNovaes, Roberto Leonan Morim
dc.creatorSilva, Bruno Alves
dc.creatorSouza, Renan de França
dc.creatorJasen, Ana Maria
dc.creatorMoratelli, Ricardo
dc.creatorRoque, André Luiz Rodrigues
dc.date2019-09-20T18:27:34Z
dc.date2019-09-20T18:27:34Z
dc.date2019
dc.date.accessioned2023-09-26T20:55:13Z
dc.date.available2023-09-26T20:55:13Z
dc.identifierRANGEL, Diana Azevedo et al. Isolation and characterization of trypanosomatids, including Crithidia mellificae, in bats from the Atlantic Forest of Rio de Janeiro, Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 7, p.1-17, July 2019.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35745
dc.identifier10.1371/journal.pntd.0007527
dc.identifier1935-2735
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866172
dc.descriptionWe studied infection by Trypanosomatidae in bats captured in two areas with different degradation levels in the Atlantic Forest of Rio de Janeiro state: Reserva Ecológica de Guapiaçu (REGUA) and Estação Fiocruz Mata Atla ˆntica (EFMA). Furthermore, we evaluated whether the diversity of trypanosomatids changes according to bat diversity and the different levels of preservation in the region. The results showed no influence of the level of preservation on bat species richness (15 and 14 species, respectively), with similar chiropterofauna and higher abundance of two common fruit-eating bat species in the tropics: Carollia perspicillata and Artibeus lituratus. Of the 181 bat specimens analyzed by LIT/Schneider hemoculture, we detected 24 infected individuals (13%), including one positive Sturnira lilium individual that was also positive by fresh blood examination. Molecular characterization using nested PCR targeting the 18 SSU rRNA-encoding gene fragment showed similar trypanosomatid infection rates in bats from the two areas: 15% in REGUA and 11% in EFMA (p = 0.46). Trypanosoma dionisii was the most frequently detected parasite (54%), followed by T. cruzi DTUs TcI and TcIV and Trypanosoma sp., in Neotropical phyllostomid bats (RNMO63 and RNMO56); mixed infections by T. dionisii/T. cruzi TcIII and T. dionisii/T. cruzi TcI were also observed. The T. cruzi DTUs TcI and TcIV are the genotypes currently involved in cases of acute Chagas disease in Brazil, and T. dionisii was recently found in the heart tissue of an infected child. Surprisingly, we also describe for the first time Crithidia mellificae, a putative monoxenous parasite from insects, infecting a vertebrate host in the Americas. Bats from the Atlantic Forest of Rio de Janeiro state harbor a great diversity of trypanosomatids, maintaining trypanosomatid diversity in this sylvatic environment.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectTripanossomatídeos
dc.subjectMorcegos
dc.subjectCrithidia mellificae
dc.subjectMata Atlântica
dc.subjectRio de janeiro
dc.subjectIsolamento
dc.subjectTrypanosomatids
dc.subjectCrithidia mellificae
dc.subjectBats
dc.subjectAtlantic Forest
dc.subjectRio de Janeiro
dc.subjectIsolation
dc.titleIsolation and characterization of trypanosomatids, including Crithidia mellificae, in bats from the Atlantic Forest of Rio de Janeiro, Brazil
dc.typeArticle


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