Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model

dc.creatorDaltro, Pamela Santana
dc.creatorBarreto, Breno Cardim
dc.creatorSilva, P G
dc.creatorChenaud Neto, Paulo
dc.creatorSousa Filho, P H F
dc.creatorSantana Neta, D
dc.creatorCarvalho, Gisele Batista
dc.creatorSilva, D N
dc.creatorParedes, Bruno Diaz
dc.creatorAlcantara, Adriano Costa de
dc.creatorFreitas, Luiz Antonio Rodrigues de
dc.creatorCouto, Ricardo David
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorSouza, Bruno Solano de Freitas
dc.creatorSoares, Milena Botelho Pereira
dc.creatorMacambira, Simone Garcia
dc.date2018-03-06T13:39:29Z
dc.date2018-03-06T13:39:29Z
dc.date2017
dc.date.accessioned2023-09-26T20:55:06Z
dc.date.available2023-09-26T20:55:06Z
dc.identifierDALTRO, P. S. et al. Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model. Cytotherapy, v. 19, p. 1176–1188, 2017.
dc.identifier1465-3249
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25070
dc.identifier10.1016/j.jcyt.2017.07.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866139
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), by Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Financiadora de Estudos e Projetos (FINEP).
dc.descriptionObesity is associated with numerous cardiac complications, including arrhythmias, cardiac fibrosis, remodeling and heart failure. Here we evaluated the therapeutic potential of mesenchymal stromal cells (MSCs) and their conditioned medium (CM) to treat cardiac complications in a mouse model of high-fat diet (HFD)-induced obesity.Methods. After obesity induction and HFD withdrawal, obese mice were treated with MSCs, CM or vehicle. Cardiac function was assessed using electrocardiography, echocardiography and treadmill test. Body weight and biochemical parameters were evaluated. Cardiac tissue was used for real time (RT)-polymerase chain reaction (PCR) and histopathologic analysis. Results/ Discussion. Characterization of CM by protein array showed the presence of different cytokines and growth factors, including chemokines, osteopontin, cystatin C, Serpin E1 and Gas 6. HFD-fed mice presented cardiac arrhythmias, altered cardiac gene expression and fibrosis reflected in physical exercise incapacity associated with obesity and diabetes. Administration of MSCs or CM improved arrhythmias and exercise capacity. This functional improvement correlated with normalization of GATA4 gene expression in the hearts of MSC- or CM-treated mice. The gene expression of connexin 43, troponin I, adiponectin, transforming growth factor (TGF) β, peroxisome proliferator activated receptor gamma (PPARγ), insulin-like growth factor 1 (IGF-1), matrix metalloproteinase-9 (MMP9) and tissue inhibitor of metalloproteinases 1 (TIMP1) were significantly reduced in MSCs, but not in CM-treated mice. Moreover, MSC or CM administration reduced the intensity of cardiac fibrosis. Conclusion. Our results suggest that MSCs and CM have a recovery effect on cardiac disturbances due to obesity and corroborate to the paracrine action of MSCs in heart disease models.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectDisfunção cardíaca
dc.subjectTerapia celular
dc.subjectCélulas estomatais mesenquimatosas
dc.subjectObesidade
dc.subjectCardiac dysfunction
dc.subjectCell therapy
dc.subjectMesenchymal stromal cells
dc.subjectObesity
dc.titleTherapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model
dc.typeArticle


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