Genetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil

dc.creatorPassaes, Caroline Pereira Bittencourt
dc.creatorBello, Gonzalo
dc.creatorLorete, Roberta S.
dc.creatorAlmeida, Sabrina E. Matos
dc.creatorJunqueira, Dennis Maletich
dc.creatorMorgado, Mariza G.
dc.date2017-08-15T13:51:35Z
dc.date2017-08-15T13:51:35Z
dc.date2009
dc.date.accessioned2023-09-26T20:54:26Z
dc.date.available2023-09-26T20:54:26Z
dc.identifierPASSAES, Carolina Pereira Bittencourt; et al. Genetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil. Infection, Genetics and Evolution, v.9, p.474-482, 9p, 2009.
dc.identifier1567-1348
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20614
dc.identifier10.1016/j.meegid.2009.01.008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865964
dc.descriptionTo evaluate the recombination profiles and evolutionary history of HIV-1 BC recombinants in Southern Brazil, 81 isolates collected in the city of Porto Alegre (Rio Grande do Sul State) from 1998 to 2006 previously subtyped as C (env-gp120/C2V3) were screened in the protease-reverse transcriptase (pr/rt), integrase and gp41 genomic regions. Detailed phylogenetic, bootscan and informative site analyses were performed to trace the subtype classification. The evolutionary rate and divergence time of the Brazilian CRF31_BC epidemic were estimated using a Bayesian Markov Chain Monte Carlo framework. Analysis of the four target regions identified: 43 isolates as "pure" subtype C, 23 as CRF31_BC, and 15 as unique BC recombinant forms (URFs_BC). Recombination breakpoints were mainly localized in the rt gene and 100% of the recombinant samples could be detected analyzing only this region. Most URFs_BC (86.7%) contained small subtype B fragments (<or=160nt) in the rt region and shared one of the recombination breakpoints with CRF31_BC. In conclusion, despite the high co-prevalence of subtypes B and C in Porto Alegre, the diversity of BC recombinant forms circulating in this region was extremely low. Most BC recombinants were CRF31_BC and URFs_BC that appeared to be second generation recombinants derived from CRF31_BC and subtype C strains, confirming the importance of this CRF in this region. The emergence of the CRF31_BC was estimated to be around 1988 (1982-1992).
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectHIV-1
dc.subjectBrasil
dc.subjectHistória da evolução
dc.subjectSubtipo C
dc.subjectRecombinantes de segunda geração
dc.subjectBrazil
dc.subjectHIV-1
dc.subjectSubtype C
dc.subjectCRF51_BC
dc.subjectSecond generation recombinants
dc.subjectEvolutionary history
dc.titleGenetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil
dc.typeArticle


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