Extracellular Serine-proteinases Isolated from Streptomyces alboniger: Partial Characterization and Effect of Aprotinin on Cellular Structure

dc.creatorLopes, Andréa
dc.creatorCoelho, Rosalie R. R.
dc.creatorMeirelles, Maria Nazareth L.
dc.creatorBranquinha, Marta Helena
dc.creatorVermelho, Alane Beatriz
dc.date2020-10-17T18:52:00Z
dc.date2020-10-17T18:52:00Z
dc.date1999
dc.date.accessioned2023-09-26T20:54:23Z
dc.date.available2023-09-26T20:54:23Z
dc.identifierLOPES, Andréa et al. Extracellular Serine-proteinases Isolated from Streptomyces alboniger: Partial Characterization and Effect of Aprotinin on Cellular Structure. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 6, p. 763-779, Nov./Dec. 1999.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44031
dc.identifier10.1590/S0074-02761999000600010
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865951
dc.descriptionStreptomyces alboniger ATCC 12461 grown in brain heart infusion (BHI) medium produced two extracellular serine-proteinases, denoted SP I and SP II, which were purified by ammonium sulfate precipitation and aprotinin-agarose affinity chromatography. SP I was purified 88,9-fold and SP II 66,7- fold, with 33.4% and 10.4% yield, respectively. The optimum pH for the proteinases activity, using α-N-p-tosyl-L-arginine-methyl ester (TAME) as substrate, was 9-10 and the optimum temperature was 37ºC. The proteolytic activity of SP I and SP II was inhibited by aprotinin and SP I was partially inhibited by leupeptin, both serine-proteinase inhibitors. S. alboniger growth in BHI-liquid medium decreased when 5 µg/ml, 10 µg/ml of aprotinin was used, being completely inhibited with 20 µg/ml and 40 µg/ml. At the ultrastructural level, aprotinin-treated S. alboniger cells showed swelling of the bacterial body and condensation of the genetic material, probably related to the inhibition of its growth.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectStreptomyces alboniger
dc.subjectAprotinina
dc.subjectCrescimento
dc.subjectSerine-proteinases
dc.subjectStreptomyces alboniger
dc.subjectSerine-proteinases
dc.subjectGrowth
dc.subjectAprotinin
dc.titleExtracellular Serine-proteinases Isolated from Streptomyces alboniger: Partial Characterization and Effect of Aprotinin on Cellular Structure
dc.typeArticle


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