Calcitonin gene-related peptide inhibits local acute inflammation and protects mice against lethal endotoxemia

dc.creatorGomes, Rachel Novaes
dc.creatorFaria Neto, Hugo Caire de Castro
dc.creatorBozza, Patrícia Torres
dc.creatorSoares, Milena Botelho Pereira
dc.creatorShoemaker, Charles B.
dc.creatorDavid, John R.
dc.creatorBozza, Marcelo Torres
dc.date2015-04-14T13:19:24Z
dc.date2015-04-14T13:19:24Z
dc.date2005
dc.date.accessioned2023-09-26T20:54:02Z
dc.date.available2023-09-26T20:54:02Z
dc.identifierGOMES, R. N. et al. Calcitonin gene-related peptide inhibits local acute inflammation and protects mice against lethal endotoxemia. Shock, v. 24, n. 6, p. 590-59, 2005.
dc.identifier1073-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9987
dc.identifier10.1097/01.shk.0000183395.29014.7c
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865848
dc.descriptionABSTRACT: Calcitonin gene-related peptide (CGRP), a potent vasodilatory peptide present in central and peripheral neurons, is released at inflammatory sites and inhibits several macrophage, dendritic cell, and lymphocyte functions. In the present study, we investigated the role of CGRP in models of local and systemic acute inflammation and on macrophage activation induced by lipopolysaccharide (LPS). Intraperitoneal pretreatment with synthetic CGRP reduces in approximately 50% the number of neutrophils in the blood and into the peritoneal cavity 4 h after LPS injection. CGRP failed to inhibit neutrophil recruitment induced by the direct chemoattractant platelet-activating factor, whereas it significantly inhibited LPSinduced KC generation, suggesting that the effect of CGRP on neutrophil recruitment is indirect, acting on chemokine production by resident cells. Pretreatment of mice with 1 mg of CGRP protects against a lethal dose of LPS. The CGRPinduced protection is receptor mediated because it is completely reverted by the CGRP receptor antagonist, CGRP 8-37. The protective effect of CGRP correlates with an inhibition of TNF-a and an induction of IL-6 and IL-10 in mice sera 90 min after LPS challenge. Finally, CGRP significantly inhibits LPS-induced TNF-a released from mouse peritoneal macrophages. These results suggest that activation of the CGRP receptor on macrophages during acute inflammation could be part of the negative feedback mechanism controlling the extension of acute inflammatory responses.
dc.formatapplication/pdf
dc.languagepor
dc.publisherLippincott Williams & Wilkins
dc.rightsopen access
dc.subjectLPS
dc.subjectCGRP
dc.subjectCytokines
dc.subjectInflammation
dc.subjectEndotoxemia
dc.titleCalcitonin gene-related peptide inhibits local acute inflammation and protects mice against lethal endotoxemia
dc.typeArticle


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