Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients’ lungs through an allergen- and interleukin-5-dependent mechanism

dc.creatorXavier-Elsas, Pedro Paulo
dc.creatorSantos-Maximiano, E.
dc.creatorQueto, T.
dc.creatorMendonça-Sales, S.
dc.creatorJoseph, D.
dc.creatorGaspar-Elsas, M. I. C.
dc.creatorVargaftig, B. B.
dc.date2015-06-11T17:09:12Z
dc.date2015-06-11T17:09:12Z
dc.date2007
dc.date.accessioned2023-09-26T20:53:03Z
dc.date.available2023-09-26T20:53:03Z
dc.identifierXAVIER-ELSAS, P. et al. Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients’ lungs through an allergen- and interleukin-5-dependent mechanism. Clinical and Experimental Allergy, Oxford, v. 37, n. 1, p. 29–38, 2007.
dc.identifier0954-7894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10795
dc.identifier10.1111/j.1365-2222.2006.02623.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865566
dc.descriptionBackground Airway challenge of ovalbumin-sensitized mice induces intrapulmonary accumulation of eosinophil progenitors. Objective To evaluate whether allergen-challenged lungs release factors promoting intrapulmonary accumulation of haemopoietic cells, and define the role of allergic lung injury, we developed a transplantation model. MethodsLung tissue from allergen-challenged, sensitized donors was ectopically grafted in syngeneic recipients, and haemopoietic progenitors inside the lungs of the recipients were quantified. ResultsIn BALB/c mice, accumulation of progenitors occurred only when: (a) donors were sensitized and airway challenged with homologous allergen; (b) and recipients were sensitized. Grafts from the appropriate donors released biologically active IL-5, which was effective in sensitized recipients. The effect of the appropriate donor–recipient combination was prevented by neutralizing anti-IL-5 antibody. Grafts from unchallenged, sensitized donors synergized with recombinant IL-5 in sensitized recipients. Unlike BALB/c, grafts from naı¨ve IL-5 transgenic CBA/Ca mice (whose lungs contained a large number of progenitors, independently of sensitization and challenge) were effective in non-transgenic, ovalbumin-sensitized recipients. ConclusionThis shows that: (a) intrapulmonary accumulation of progenitors is independent of immunological injury; (b) grafts systemically release IL-5, which is required for progenitor accumulation in the recipients’ lungs; (c) and sensitization is required for full responsiveness to IL-5 and for generation of lung-derived signals that synergize with IL-5.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsrestricted access
dc.subjectEosinophils
dc.subjectHaematopoiesis
dc.subjectLung
dc.subjectTransplantation
dc.subjectEosinófilos
dc.subjectHematopoese
dc.subjectPulmão
dc.subjectTransplante
dc.titleEctopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients’ lungs through an allergen- and interleukin-5-dependent mechanism
dc.typeArticle


Este ítem pertenece a la siguiente institución