Genetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: high diversity of HIV genotypes.

dc.creatorMonteiro, Joana Paixão
dc.creatorAlcantara, Luiz Carlos Júnior
dc.creatorOliveira, Tulio de
dc.creatorOliveira, Antonio Marcos
dc.creatorMelo, Marco Antônio Gomes
dc.creatorBrites, Carlos
dc.creatorCastro Filho, Bernardo Galvão
dc.date2014-07-28T13:02:22Z
dc.date2014-07-28T13:02:22Z
dc.date2009
dc.date.accessioned2023-09-26T20:52:54Z
dc.date.available2023-09-26T20:52:54Z
dc.identifierMONTEIRO, J. P. et al. Genetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: high diversity of HIV genotypes. Journal of Medical Virology, v. 81, n. 3, p. 391–399, 2009.
dc.identifier1096-9071
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8087
dc.identifier10.1002/jmv.21414
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865518
dc.descriptionThe HIV-1 genetic variability in Bahia state, Brazil, was investigated. DNA samples from 229 and 213 HIV-1-infected individuals were analyzed using the heteroduplex mobility assay (HMA) in gag and env fragments, respectively. One hundred seventy-five samples were characterized in both genes. Thirty-two subtype F and BF recombinant viruses were sequenced and analyzed by phylogenetic methods. The combination of HMA and sequencing results showed that seven different HIV-1 genotypes comprised this sample: 147 (84%) B/B, 4 (2.3%) F/F, 3 (1.7%) B/F, 1 (0.6%) F/B, 1 (0.6%) F/D, 1 (0.6%) BF/F, and 18 (10.3%) BF/B. A significant divergence was observed between these two techniques results (84.4%). This is explained by the low accuracy of the HMA for detecting recombinant viruses. These recombinants were unrelated to CRF12, while two sequences were related to CRF28 and CRF29. Nineteen BF mosaics shared the same gag breakpoint. In conclusion, the use of HMA may be inappropriate in regions where different subtypes are co-circulating. Subtype B is the most common genotype, however, an increased prevalence (13.1%) of different BF variants and a potentially new CRF suggest that recombination is occurring frequently in Bahia. These viruses were associated with women infected heterosexually. Finally, this study identified the presence of an F/D recombinant HIV-1 in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley-Liss, INC
dc.rightsopen access
dc.subjectHIV-1
dc.subjectEpidemiology
dc.subjectPhylogeny
dc.subjectVariação Genética
dc.subjectInfecções por HIV/epidemiologia
dc.subjectInfecções por HIV/virologia
dc.subjectHIV-1/classificação
dc.subjectHIV-1/genética
dc.subjectAdulto
dc.subjectBrasil/epidemiologia
dc.subjectAnálise por Conglomerados
dc.subjectDNA Viral/genética
dc.subjectHIV-1/isolamento & purificação
dc.subjectHIV-1/isolamento & purificação
dc.subjectFeminino
dc.subjectGenótipo
dc.subjectAnálise Heteroduplex
dc.subjectHumanos
dc.subjectMasculino
dc.subjectDados de Sequência Molecular
dc.subjectAnálise de Sequência de DNA
dc.subjectFilogenia
dc.subjectHomologia de Sequência
dc.subjectProdutos do Gene env do Vírus da Imunodeficiência Humana/genética
dc.titleGenetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: high diversity of HIV genotypes.
dc.typeArticle


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