Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.

dc.creatorFlannery, Brendan
dc.creatorCosta, Dirceu Joaquim
dc.creatorCarvalho, Fernanda Pinheiro
dc.creatorGuerreiro, Hygia
dc.creatorMatsunaga, James
dc.creatorSilva, Emilson Domingos da
dc.creatorFerreira, Antonio Gomes Pinto
dc.creatorRiley, Lee Woodland
dc.creatorReis, Mitermayer Galvão dos
dc.creatorHaake, David A
dc.creatorKo, Albert Icksang
dc.date2014-05-02T16:45:58Z
dc.date2014-05-02T16:45:58Z
dc.date2001
dc.date.accessioned2023-09-26T20:51:50Z
dc.date.available2023-09-26T20:51:50Z
dc.identifierFLANNERY, B. et al. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. Journal of Clinical Microbiology, v. 39, n. 9, p. 3303-3310, 2001 .
dc.identifier0095-1137
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7569
dc.identifier10.1128/JCM.39.9.3303–3310.2001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865212
dc.descriptionThere is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96% specificity. In paired sera from 50 cases of leptospirosis confirmed by the microscopic agglutination test, immunoglobulin G (IgG) but not IgM reacted with the recombinant leptospiral proteins. The recombinant LipL32 IgG ELISA had the highest sensitivities in the acute (56%) and convalescent (94%) phases of leptospirosis. ELISAs based on recombinant OmpL1, LipL41, and Hsp58 had sensitivities of 16, 24, and 18% during the acute phase and 72, 44, and 32% during convalescence, respectively. Compared to sera from healthy individuals, patient sera did not react significantly with recombinant LipL36 (P > 0.05). Recombinant LipL32 IgG ELISA demonstrated 95% specificity among 100 healthy individuals, and specificities ranging from 90 to 97% among 30 dengue patients, 30 hepatitis patients, and 16 patients with diseases initially thought to be leptospirosis. Among 39 Venereal Disease Research Laboratory test-positive individuals and 30 Lyme disease patients, 13 and 23% of sera, respectively, reacted positively with the rLipL32 antigen. These findings indicate that rLipL32 may be an useful antigen for the serodiagnosis of leptospirosis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectAnticorpos Antibacterianos/sangue
dc.subjectAntígenos de Bactérias/imunologia
dc.subjectEnsaio de Imunoadsorção Enzimática/métodos
dc.subjectLeptospira/imunologia
dc.subjectLeptospirose/diagnóstico
dc.subjectAntígenos de Bactérias/genética
dc.subjectEnsaio de Imunoadsorção Enzimática/normas
dc.subjectHumanos
dc.subjectImunoglobulina G/sangue
dc.subjectLipoproteínas/genética
dc.subjectLipoproteínas/imunologia
dc.subjectProteínas Recombinantes/genética
dc.subjectSensibilidade e Especificidade
dc.titleEvaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
dc.typeArticle


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