Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis

dc.creatorSilveira Júnior, Lenilton Silva da
dc.creatorSilva, Franklin Souza
dc.creatorPereira, Bernardo Acácio Santini
dc.creatorFinkelstein, Léa Cysne
dc.creatorCavalcanti Júnior, Geraldo Barroso
dc.creatorAlves, Carlos Roberto
dc.date2017-07-06T15:29:06Z
dc.date2017-07-06T15:29:06Z
dc.date2017
dc.date.accessioned2023-09-26T20:51:39Z
dc.date.available2023-09-26T20:51:39Z
dc.identifierSILVEIRA JUNIOR, Lenilton Silva da; et al. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis. BioMed Research International, Volume 2017, Article ID 9089748, 9p, 2017.
dc.identifier2314-6141
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/19743
dc.identifier10.1155/2017/9089748
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865160
dc.descriptionA surface plasmon resonance- (SPR-) based recognition method applying H-2 L(d):Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with Leishmania (Leishmania) amazonensis is described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2 L(d):Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B of L. (L.) amazonensis. In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2 L(d) at neutral pH, with a Gibbs free energy ΔG° < 0. The assayed DimerX:peptide complexes presented the property of attaching to distinct T lymphocytes subsets, obtained from experimentally infected BALB/c mice, in each week of infection, thus indicating a temporal variation in specific T lymphocytes populations, each directed to a different COOH-terminal region-derived peptide. The experimental design proposed herein is an innovative approach for cellular immunology studies of a neglected disease, providing a useful tool for the analysis of specific T lymphocytes subsets.
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.rightsopen access
dc.subjectLeishmania
dc.subjectDoenças negligenciadas
dc.subjectEstudos de imunologia celular
dc.subjectSubconjuntos específicos de linfócitos T
dc.subjectLeishmania (Leishmania) amazonensis
dc.subjectneglected disease
dc.subjectcellular immunology studies
dc.subjectspecific T lymphocytes subsets
dc.titleExploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis
dc.typeArticle


Este ítem pertenece a la siguiente institución