Synthetic Antigens Derived from Plasmodium falciparum Sporozoite, Liver, and Blood Stages: Naturally Acquired Immune Response and Human Leukocyte Antigen Associations in Individuals Living in a Brazilian Endemic Area

dc.creatorPratt-Riccio, Lilian Rose
dc.creatorDe Souza Perce-Da-Silva, Daiana
dc.creatorDa Costa Lima-Junior, Josué
dc.creatorPratt Riccio, Evelyn Kety
dc.creatorRibeiro-Alves, Marcelo
dc.creatorSantos, Fátima
dc.creatorArruda, Mercia
dc.creatorCamus, Daniel
dc.creatorDruilhe, Pierre
dc.creatorOliveira-Ferreira, Joseli
dc.creatorDaniel-Ribeiro, Cláudio Tadeu
dc.creatorBanic, Dalma Maria
dc.date2018-04-17T15:30:47Z
dc.date2018-04-17T15:30:47Z
dc.date2017
dc.date.accessioned2023-09-26T20:51:23Z
dc.date.available2023-09-26T20:51:23Z
dc.identifierPRATT-RICCIO, Lilian Rose, et al. Synthetic Antigens Derived from Plasmodium falciparum Sporozoite, Liver, and Blood Stages: Naturally Acquired Immune Response and Human Leukocyte Antigen Associations in Individuals Living in a Brazilian Endemic Area. American Journal of Tropical Medicine and Hygiene, v. 97, n. 5, p. 1581-1592, 2017.
dc.identifier0002-9637
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25904
dc.identifier10.4269/ajtmh.17-0359
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865098
dc.descriptionPeptide vaccine strategies using Plasmodium-derived antigens have emerged as an attractive approach against malaria. However, relatively few studies have been conducted with malaria-exposed populations from non-African countries. Herein, the seroepidemiological profile against Plasmodium falciparum of naturally exposed individuals from a Brazilian malaria-endemic area against synthetic peptides derived from vaccine candidates circumsporozoite protein (CSP), liver stage antigen-1 (LSA-1), erythrocyte binding antigen-175 (EBA-175), and merozoite surface protein-3 (MSP-3) was investigated. Moreover, human leukocyte antigen (HLA)-DRB1* and HLA-DQB1* were evaluated to characterize genetic modulation of humoral responsiveness to these antigens. The study was performed using blood samples from 187 individuals living in rural malaria-endemic villages situated near Porto Velho, Rondônia State. Specific IgG and IgM antibodies and IgG subclasses were detected by enzyme-linked immunosorbent assay, and HLA-DRB1* and HLA-DQB1* low-resolution typing was performed by PCR-SSP. All four synthetic peptides were broadly recognized by naturally acquired antibodies. Regarding the IgG subclass profile, only CSP induced IgG1 and IgG3 antibodies, which is an important fact given that the acquisition of protective immunity appears to be associated with the cytophilicity of IgG1 and IgG3 antibodies. HLA-DRB1*11 and HLA-DQB1*7 had the lowest odds of responding to EBA-175. Our results showed that CSP, LSA-1, EBA, and MSP-3 are immunogenic in natural conditions of exposure and that anti-EBA antibody responses appear to be modulated by HLA class II antigens.
dc.description2500-12-31
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectPlasmodium
dc.subjectVacinas Sintéticas
dc.subjectMalária
dc.subjectPlasmodium
dc.subjectMalária
dc.subjectVacinas Sintéticas
dc.titleSynthetic Antigens Derived from Plasmodium falciparum Sporozoite, Liver, and Blood Stages: Naturally Acquired Immune Response and Human Leukocyte Antigen Associations in Individuals Living in a Brazilian Endemic Area
dc.typeArticle


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