Comparison of four molecular approaches to identify Candida parapsilosis complex species

dc.creatorBarbedo, Leonardo Silva
dc.creatorFigueiredo-Carvalho, Maria Helena Galdino
dc.creatorMuniz, Mauro de Medeiros
dc.creatorZancopé-Oliveira, Rosely Maria
dc.date2019-01-24T11:18:48Z
dc.date2019-01-24T11:18:48Z
dc.date2017
dc.date.accessioned2023-09-26T20:51:06Z
dc.date.available2023-09-26T20:51:06Z
dc.identifierBARBEDO, Leonardo Silva et al. Comparison of four molecular approaches to identify Candida parapsilosis complex species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 3, p. 214-219, Mar. 2017.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31274
dc.identifier10.1590/0074-02760160412
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865012
dc.descriptionSince the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto Oswaldo Cruz
dc.rightsopen access
dc.subjectCandida parapsilosis complex
dc.subjectCandida orthopsilosis
dc.subjectCandida metapsilosis
dc.subjectCandidaemia
dc.subjectDNA
dc.subjectPCR
dc.titleComparison of four molecular approaches to identify Candida parapsilosis complex species
dc.typeArticle


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