Inhibition of Efflux Pumps by Monoterpene (α-Pinene) and Impact on Staphylococcus aureus Resistance to Tetracycline and Erythromycin

dc.creatorFreitas, Priscilla Ramos
dc.creatorAraújo, Ana Carolina Justino de
dc.creatorBarbosa, Cristina R.
dc.creatorMuniz, Débora F.
dc.creatorTintino, Saulo R.
dc.creatorRibeiro Filho, Jaime
dc.creatorSiqueira Júnior, José P.
dc.creatorBarbosa Filho, José Maria
dc.creatorSousa, Gabriela R. de
dc.creatorCoutinho, Henrique D. M.
dc.date2020-09-24T14:38:45Z
dc.date2020-09-24T14:38:45Z
dc.date2020
dc.date.accessioned2023-09-26T20:50:58Z
dc.date.available2023-09-26T20:50:58Z
dc.identifierFREITAS, Priscilla Ramos et al. Inhibition of Efflux Pumps by Monoterpene (α-Pinene) and Impact on Staphylococcus aureus Resistance to Tetracycline and Erythromycin. Current Drug Metabolism, v. 21, p. 1-4, 2020.
dc.identifier1389-2002
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43604
dc.identifier10.2174/1389200221999200730212721
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864975
dc.descriptionRibeiro-Filho, Jaime. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Documento protegido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.
dc.descriptionAbstract: Introduction: Infectious diseases have been responsible for an increasing number of deaths worldwide. Staphylococcus aureus has been recognized as one of the most notable causative agents of severe infections, while efflux pump (EP) expression is one of the main mechanisms associated with S. aureus resistance to antibiotics. Objective: This study aimed to investigate the potential of α-pinene as an efflux pump inhibitor in species of S. aureus carrying the TetK and MrsA proteins. Methods: The minimum inhibitory concentrations (MIC) of α-pinene and other efflux pump inhibitors were assessed using serial dilutions of each compound at an initial concentration above 1024 μg/mL. Solutions containing culture medium and bacterial inoculums were prepared in test tubes and subsequently transferred to 96-well microdilution plates. The modulation of ethidium bromide (EtBr) and antibiotics (tetracycline and erythromycin) was investigated through analysis of the modification in their MICs in the presence of a subinhibitory concentration of α-pinene (MIC/8). Wells containing only culture medium and bacterial inoculums were used as negative control. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) was used as a positive control. Results: The MIC of ethidium bromide against S. aureus strains RN-4220 and IS-58 was reduced by association with α-pinene. This monoterpene potentiated the effect of tetracycline against the IS-58 strain but failed in modulating the antibacterial effect of erythromycin against RN-4220, suggesting a selective inhibitory effect on the TetK EP by α- pinene. Conclusion: In conclusion, α-pinene has promising effects against S.aureus strains, which should be useful in the combat of antibacterial resistance associated with EP expression. Nevertheless, further research is required to fully characterize its molecular mechanism of action as an EP inhibitor
dc.formatapplication/pdf
dc.languageeng
dc.publisherBentham Science Publishers
dc.rightsrestricted access
dc.subjectMonoterpeno
dc.subjectStaphylococcus aureus
dc.subjectProteína
dc.subjectResistência a antibióticos
dc.subjectInibidores
dc.subjectα-pinene
dc.subjectMonoterpene
dc.subjectStaphylococcus aureus
dc.subjectTetK protein
dc.subjectMrsA protein
dc.subjectEfflux pump inhibitors
dc.titleInhibition of Efflux Pumps by Monoterpene (α-Pinene) and Impact on Staphylococcus aureus Resistance to Tetracycline and Erythromycin
dc.typeArticle


Este ítem pertenece a la siguiente institución