The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence

dc.creatorRistow, Paula
dc.creatorBourhy, Pascale
dc.creatorMcBride, Flávia Weykamp da Cruz
dc.creatorFigueira, Cláudio Pereira
dc.creatorHuerre, Michel
dc.creatorAve, Patrick
dc.creatorGirons, Isabelle Saint
dc.creatorKo, Albert Icksang
dc.creatorPicardeau, Mathieu
dc.date2015-11-06T13:30:12Z
dc.date2015-11-06T13:30:12Z
dc.date2007
dc.date.accessioned2023-09-26T20:49:55Z
dc.date.available2023-09-26T20:49:55Z
dc.identifierRISTOW, P. et al. The OmpA-Like protein Loa22 is essential for Leptospiral virulence. Plos Pathogens, v. 3, n. 7, p. e97, 2007.
dc.identifier1553-7366
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12132
dc.identifier10.1371/journal.ppat.0030097
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864662
dc.descriptionPathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity. The resulting mutant did not express Loa22 and was attenuated in virulence in the guinea pig and hamster models of leptospirosis, whereas the genetically complemented strain was restored in Loa22 expression and virulence. Our results show that Loa22 was expressed during host infection and exposed on the cell surface. Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeptospirose
dc.subjectLeptospira
dc.subjectLeptospira interrogans
dc.subjectVirulência
dc.subjectAnimal
dc.subjectHamaster
dc.titleThe OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
dc.typeArticle


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