The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells

dc.creatorRobert, Anny Waloski
dc.creatorGomes, Felipe Azevedo
dc.creatorRode, Michele Patricia
dc.creatorSilva, Maiara Marques da
dc.creatorVeleirinho, Maria Beatriz da Rocha
dc.creatorMaraschin, Marcelo
dc.creatorHayashi, Leila
dc.creatorCalloni, Giordano Wosgrau
dc.creatorStimamiglio, Marco Augusto
dc.date2019-07-15T17:40:34Z
dc.date2019-07-15T17:40:34Z
dc.date2019
dc.date.accessioned2023-09-26T20:49:29Z
dc.date.available2023-09-26T20:49:29Z
dc.identifierROBERT, Anny Waloski et al. The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells. Journal of Tissue Engineering, v. 10, p. 1-10, 2019.
dc.identifier2041-7314
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/34110
dc.identifier10.1177/2041731419833391
dc.identifier2041-7314
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864533
dc.descriptionMultipotent stromal cells stimulate skin regeneration after acute or chronic injuries. However, many stem cell therapy protocols are limited by the elevated number of cells required and poor cell survival after transplantation. Considering that the beneficial effects of multipotent stromal cells on wound healing are typically mediated by paracrine mechanisms, we examined whether the conditioned medium from skin-derived multipotent stromal cells would be beneficial for restoring the skin structure of mice after wounding. A proteomic characterization of skin-derived multipotent stromal cell-conditioned medium was performed, and the angiogenic function of this secretome was investigated in vitro using an endothelial cell tube formation assay. We then applied the skin-derived multipotent stromal cell-conditioned medium directly to full-thickness excisional wounds or embedded it into carrageenan or poly(vinyl alcohol) hydrogels to monitor tissue regeneration in mice. Biological processes related to wound healing and angiogenesis were highlighted by the analysis of the skin-derived multipotent stromal cell secretome, and a pro-angiogenic capacity for promoting tubule-like structures was first confirmed in vitro. Skin wounds treated with skin-derived multipotent stromal cell-conditioned medium also displayed increased angiogenesis, independently of the association of the conditioned medium with hydrogels. However, improvements in wound closure and epidermis or decreased inflammatory cell presence were not observed. Hence, the use of the secretome obtained from human skin-derived multipotent stromal cells may be a potential strategy to aid the natural skin repair of full-thickness lesions mainly based on its pro-angiogenic properties.
dc.formatapplication/pdf
dc.languagepor
dc.publisherSAGE Publications
dc.rightsopen access
dc.subjectSecretome
dc.subjectStromal Cells
dc.subjectWound Healing
dc.subjectPhysiologic Angiogenesis
dc.subjectCélulas del Estroma
dc.subjectCicatrización de Heridas
dc.subjectAngiogénesis fisiológica
dc.subjectCélulas Estromais
dc.subjectCicatrização
dc.subjectAngiogênese Fisiológica
dc.titleThe skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
dc.typeArticle


Este ítem pertenece a la siguiente institución