Burkholderia cenocepacia, B. multivorans, B. ambifaria and B. vietnamiensis isolates from cystic fibrosis patients have different profiles of exoenzyme production

dc.creatorCarvalho, Ana Paula D´Allicourt
dc.creatorVentura, Grasiella Maria Carvalho
dc.creatorPereira, Carolina Borges
dc.creatorLeão, Robson Souza
dc.creatorFolescu, Tânia Wrobel
dc.creatorHiga, Laurinda Yoko Shinzato
dc.creatorTeixeira, Lucia Martins
dc.creatorPlotkowski, Maria Cristina Maciel
dc.creatorMerquior, Vânia Lucia Carreira
dc.creatorAlbano, Rodolpho Mattos
dc.creatorMarques, Elizabeth Andrade
dc.date2010-08-23T16:00:18Z
dc.date2010-08-23T16:00:18Z
dc.date2007
dc.date.accessioned2023-09-26T20:49:26Z
dc.date.available2023-09-26T20:49:26Z
dc.identifierCARVALHO, Ana Paula D´Allicourt et al. Burkholderia cenocepacia, B. multivorans, B. ambifaria and B. vietnamiensis isolates from cystic fibrosis patients have different profiles of exoenzyme production. APMIS, Conpenhagen, v. 115, n. 4, p. 311-318, Apr. 2007.
dc.identifier0903-4641
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/230
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864520
dc.descriptionKnowledge about the virulence mechanisms of species from the Burkholderia cepacia complex (BCC) is still limited. The genomovar heterogeneity and production of different virulence factors are likely to contribute to the variation in the clinical outcome observed in BCC-infected cystic fibrosis (CF) patients. Therefore, in this study we investigated the genetic polimorphism, the presence of genetic makers associated with virulence and transmissibility in BCC, and the profile of exoenzyme production of 59 BCC isolates obtained from 59 CF patients attending the reference CF centre in Rio de Janeiro, Brazil. The DNA sequence analyses of the recA gene allowed us to identify 40 of these 59 BCC species as being B. cenocepacia, 9 as B. vietnamiensis, 6 as B. multivorans and 4 as B. ambifaria. The assessment of the bacterial genetic polymorphism by PFGE revealed that B. cenocepacia and the B. multivorans isolates belonged to four and two different PFGE profiles with prevalence of two clones, A and B, respectively. All B. vietnamiensis and B. ambifaria belonged to only one PFGE profile (J and E, respectively). None of the isolates exhibited the genetic markers cblA and BCESM, assessed by polymerase chain reaction. In contrast, the profile of enzymatic activity, assessed by phenotypic methods, differed among the BCC species: protease activity was detected only in B. cenocepacia and B. ambifaria isolates, whereas only B. vietnamiensis isolates produced hemolysin. Although the phospholipase C activity was similar among the different species, the level of lipase activity produced by B. multivorans was higher than in the other species. We speculate that the differential characteristics of exoenzyme production may account for the differences in the pathogenic potentials of each BCC species.
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectBurkholderia Cepacia Complex
dc.subjectCystic Fibrosis
dc.subjectVirulence Factors
dc.subjectTransmissibility Markers
dc.subjectGenetic Polymorphism
dc.subjectBurkholderia - isolamento & purificação
dc.subjectInfecções por Burkholderia - microbiologia
dc.subjectBurkholderia Cepacia - isolamento & purificação
dc.subjectFibrose Cística - microbiologia
dc.subjectPneumopatias - microbiologia
dc.titleBurkholderia cenocepacia, B. multivorans, B. ambifaria and B. vietnamiensis isolates from cystic fibrosis patients have different profiles of exoenzyme production
dc.typeArticle


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