Leishmania infantum-induced primary and challenge infections in rhesus monkeys (Macaca mulatta): a primate model for visceral leishmaniasis.

dc.creatorPorrozzi, Renato
dc.creatorPereira, Miriam Sales
dc.creatorTeva, Antonio
dc.creatorVolpini, Angela Cristina
dc.creatorPinto, Marcelo Alves
dc.creatorMarchevskyc, Renato Sergio
dc.creatorBarbosa Junior, Aryon de Almeida
dc.creatorGrimaldi Junior, Gabriel
dc.date2016-03-02T14:19:30Z
dc.date2016-03-02T14:19:30Z
dc.date2006
dc.date.accessioned2023-09-26T20:49:09Z
dc.date.available2023-09-26T20:49:09Z
dc.identifierPORROZZI, R. et al. Leishmania infantum-induced primary and challenge infections in rhesus monkeys (Macaca mulatta): a primate model for visceral leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 100, p. 926-937, 2006.
dc.identifier0035-9203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12943
dc.identifier10.1016/j.trstmh.2005.11.005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864441
dc.descriptionVisceral leishmaniasis (VL) was experimentally induced in rhesus macaques (Macaca mulatta) by intravenously inoculating 2×107 amastigotes/kg of body weight of Leishmania infantum. The macaques developed a systemic disease showing characteristic features of human VL such as fever, diarrhoea, body weight loss, anaemia, hypergammaglobulinaemia and transient lymphocytosis, as well as lymph node, liver and/or spleen enlargement. Nine weeks after infection, one primate showed pronounced weight loss, became moribund and was euthanized. The necropsy findings included granulomas composed of parasite-containing macrophages, lymphocytes and plasma cells in the liver, spleen and lymph nodes. The remaining macaques had a sustained course of infection but developed a mild-to-moderate illness that subsequently showed evidence of self-cure. Of note, pathological findings included a typical cell-mediated immunity-induced granulomatous reaction that had an effect on the control of parasite replication. All infected monkeys responded with increased production of anti-Leishmania-specific IgG antibodies. Despite the fact that clinical resistance to L. infantum was not consistently associated with a parasite-specific cell-mediated immune response, drug-cured macaques from the primary infection acquired immunity to homologous re-infection. These findings point to the feasibility of using the L. infantum macaque model for pre-clinical evaluation of novel chemotherapeutics or vaccine candidates for human VL.
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.rightsopen access
dc.subjectVisceral leishmaniasis
dc.subjectLeishmania infantum
dc.subjectMacaca mulatta
dc.subjectImmune responses
dc.subjectHistopathology
dc.subjectTreatment
dc.subjectModelos Animais de Doenças
dc.subjectLeishmania infantum/imunologia
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectFormação de Anticorpos
dc.subjectAntígenos de Protozoários/sangue
dc.subjectDNA de Protozoário/análise
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectDoenças Hematológicas/parasitologia
dc.subjectImunidade Celular
dc.subjectLeishmaniose Visceral/sangue
dc.subjectMacaca mulatta
dc.titleLeishmania infantum-induced primary and challenge infections in rhesus monkeys (Macaca mulatta): a primate model for visceral leishmaniasis.
dc.typeArticle


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