Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction

dc.creatorAbud, Ana Paula Ressetti
dc.creatorPaschoal, Ariane Caroline Campos
dc.creatorKuligovski, Crisciele
dc.creatorCaruso, Rodrigo Rêgo Barros
dc.creatorDallagiovanna, Bruno
dc.creatorAguiar, Alessandra Melo de
dc.date2021-12-14T13:28:59Z
dc.date2021-12-14T13:28:59Z
dc.date2021
dc.date.accessioned2023-09-26T20:47:48Z
dc.date.available2023-09-26T20:47:48Z
dc.identifierABUD, Ana Paula Ressetti et al. Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction. MethodsX, v.8, n. 101515, p. 1–22, 2021.
dc.identifier2215-0161
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50405
dc.identifier10.1016/j.mex.2021.101515
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864054
dc.descriptionIn vitro stem cell models are used as alternatives to animal models and are important tools for cytotoxicity studies. Researchers can determine the effects of test substances on human cells by evaluating cell viability and differentiation. Here, we describe an in vitro model to quantify adipogenesis based on the Nile red staining of specific lipid droplets and the emission of basic lipids from human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) in the presence of test substances. This assay allows for the prediction of toxicity based on the inhibition of adipogenesis in vitro in a 96-well format. The differentiation of a progenitor cell into a specialized cell, the adipocyte, is easy to monitor and quantify, making this a simple assay. The fluorescence staining of nuclei and lipid droplets is measured after 14 days of cell differentiation to determine cell number and assess cell differentiation using high-content imaging analysis, thus allowing for the identification of chemicals that impact differentiation. We also describe a protocol to assess adipocyte differentiation by fluorescence intensity using a multiplate reader.
dc.formatapplication/pdf
dc.languagepor
dc.publisherElsevier
dc.rightsopen access
dc.subjectStem Cells
dc.subjectCell Differentiation
dc.subjectCytotoxicity
dc.subjectAdipogenesis
dc.subjectCélulas Madre
dc.subjectDiferenciación Celular
dc.subjectCitotoxicidad
dc.subjectAdipogénesis
dc.subjectCellules souches
dc.subjectDifférenciation cellulaire
dc.subjectCytotoxicité
dc.subjectAdipogenèse
dc.subjectCélulas-Tronco
dc.subjectDiferenciação Celular
dc.subjectCitotoxicidade
dc.subjectAdipogenia
dc.titleUsing inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
dc.typeArticle


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