Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays

dc.creatorCastro Junior, José Geraldo
dc.creatorFreire, Mariana Lourenço
dc.creatorCampos, Samantha Priscila Silva
dc.creatorScopel, Kezia K. G
dc.creatorPorrozzi, Renato
dc.creatorSilva, Edmilson Domingos da
dc.creatorColombo, Fábio A
dc.creatorSilveira, Rita de Cássia Viveiros da
dc.creatorMarques, Marcos José
dc.creatorCoimbra, Elaine Soares
dc.date2015-06-08T14:01:52Z
dc.date2015-06-08T14:01:52Z
dc.date2014
dc.date.accessioned2023-09-26T20:47:11Z
dc.date.available2023-09-26T20:47:11Z
dc.identifierCASTRO JUNIOR, José Geraldo et al. Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays. Rev. Inst. Med. Trop. Sao Paulo, v.56, n.3, p.225-229, May-June, 2014.
dc.identifier0036-4665
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10699
dc.identifier10.1590/S0036-46652014000300008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863860
dc.descriptionIn Brazil, domestic dogs are branded as the primary reservoir for zoonotic visceral leishmaniasis, due to the clear positive correlation observed between human and canine infection rates. This study aimed to carry out a serological survey of canine visceral leishmaniasis (CVL) in dogs housed at a public kennel in the municipality of Juiz de Fora, Minas Gerais State, Brazil, using the immunochromatographic TR DPP® CVL rapid test. Additionally, conventional and/or real time PCR assay was used to detect and confirm L. infantum infection in the DPP positive dogs only. Of the 400 dogs studied, most did not present clinical signs for CVL (p < 0.05), and fifteen (3.8%) were seropositive in the DPP test. There was no statistically significant difference between the DPP seropositive dogs and the clinical signs of the disease (p > 0.05). Both conventional and real time PCR tests confirmed L. infantum infection in nine (75.0%) of the twelve DPP seropositive dogs that remained alive during the follow-up period. This study is the first seroepidemiologic survey of CVL held in the city of Juiz de Fora, and the results reinforce the idea that this disease is currently in a process of expansion and urbanization in Brazil. Furthermore, this study highlights the use of the DPP test as an alternative for diagnosing CVL in large and mid-sized cities, due to its ease of implementation.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto de Medicina Tropical de São Paulo
dc.rightsopen access
dc.subjectCanine visceral leishmaniasis
dc.subjectSerological diagnosis
dc.subjectImmunochromatographic diagnosis
dc.subjectDPP
dc.subjectPCR diagnosis
dc.subjectLeishmaniose Visceral
dc.subjectCães
dc.subjectDiagnóstico
dc.subjectReação em Cadeia da Polimerase
dc.titleEvidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
dc.typeArticle


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