dc.creatorAlves, F. A.
dc.creatorSouza, M. T.
dc.creatorGonçalves, E. C.
dc.creatorSchneider, M. P. C.
dc.creatorMarinho, A. M.
dc.creatorMuniz, J. A. P. C.
dc.creatorFragoso, S. P.
dc.creatorKrieger, M. A.
dc.creatorGoldenberg, S.
dc.creatorDaniel-Ribeiro, Cláudio Tadeu
dc.creatorCarvalho, L. J. M.
dc.date2018-04-17T17:38:33Z
dc.date2018-04-17T17:38:33Z
dc.date2010
dc.date.accessioned2023-09-26T20:46:21Z
dc.date.available2023-09-26T20:46:21Z
dc.identifierALVES, F. A. et al. DNA sequencing of 13 cytokine gene fragments of Aotus infulatus and Saimiri sciureus, two non-human primate models for malaria. Cytokine, v.52, p.151-155, 2010.
dc.identifier1043-4666
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25913
dc.identifier10.1016/j.cyto.2010.09.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863606
dc.descriptionAotus and Saimiri are non-human primate models recommended by the World Health Organization for experimental studies in malaria, especially for vaccine pre-clinical trials. However, research using these primates is hindered by the lack of specific reagents to evaluate immune responses to infection or vaccination. As a step toward developing molecular tools for cytokine expression studies in these species, primer pairs for 18 cytokine gene fragments were designed based on human DNA sequences and used to amplify the corresponding genes in Aotus infulatus and Saimiri sciureus genomic DNA samples. IFNγ, TNFα, LTA, IL2, IL3, IL4, IL5, IL6, IL10, IL12, IL13, CSF2 and TGFβ2 gene fragments were amplified and sequenced. Primer pairs for IL8, IL17, IL18, IL27 and MIF failed to generate amplification products. When compared to the available corresponding human and non-human primate sequences, most--except IL3 and IL4--showed identity degrees above 90%. Small variations in sequence can help to explain the failure to amplify certain genes or the amplification only at lower annealing temperatures as compared to human DNA samples for several primer pairs. The sequences made available provide the basis for designing molecular tools such as primers for real time PCR specific for A. infulatus and/or S. sciureus. The nucleotide sequences reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned accession numbers DQ985386 to DQ985389, DQ989356 to DQ989369, FJ89020 to FJ89024, and FJ89029.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectMalária
dc.subjectCitocinas
dc.subjectsistema imunológico
dc.subjectAotus trivirgatus
dc.subjectSaimiri
dc.subjectAotus
dc.subjectSaimiri
dc.subjectImmune system
dc.subjectCytokines
dc.subjectMalaria
dc.titleDNA sequencing of 13 cytokine gene fragments of Aotus infulatus and Saimiri sciureus, two non-human primate models for malaria
dc.typeArticle


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