The live-attenuated yellow fever vaccine 17D induces broad and potent T cell responses against several viral proteins in Indian rhesus macaques--implications for recombinant vaccine design

dc.creatorMudd, Philip A.
dc.creatorPiaskowski, Shari M.
dc.creatorNeves, Patricia C. Costa
dc.creatorRudersdorf, Richard
dc.creatorKolar, Holly L.
dc.creatorEernisse, Christopher M.
dc.creatorWeisgrau, Kim L.
dc.creatorSantana, Marlon G. Veloso de
dc.creatorWilson, Nancy A.
dc.creatorBonaldo, Myrna C.
dc.creatorGaller, Ricardo
dc.creatorRakasz, Eva G.
dc.creatorWatkins, David I.
dc.date2017-09-12T11:02:12Z
dc.date2017-09-12T11:02:12Z
dc.date2010
dc.date.accessioned2023-09-26T20:46:19Z
dc.date.available2023-09-26T20:46:19Z
dc.identifierMUDD, Philip A. et al. The live-attenuated yellow fever vaccine 17D induces broad and potent T cell responses against several viral proteins in Indian rhesus macaques – implications for recombinant vaccine design. Immunogenetics, v.62, n.9, p.593-600, Sept. 2010.
dc.identifier0093-7711
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20950
dc.identifier10.1007/s00251-010-0461-0
dc.identifier1432-1211
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863597
dc.descriptionThe yellow fever vaccine 17D (YF17D) is one of the most effective vaccines. Its wide use and favorable safety profile make it a prime candidate for recombinant vaccines. It is believed that neutralizing antibodies account for a large measure of the protection afforded to YF17D-vaccinated individuals, however cytotoxic T lymphocyte (CTL) responses have been described in the setting of YF17D vaccination. YF17D is an ssRNA flavivirus that is translated as a full-length polyprotein, several domains of which pass into the lumen of the endoplasmic reticulum (ER). The processing and presentation machinery for MHC class I-restricted CTL responses favor cytoplasmic peptides that are transported into the ER by the transporter associated with antigen presentation proteins. In order to inform recombinant vaccine design, we sought to determine if YF17D-induced CTL responses preferentially targeted viral domains that remain within the cytoplasm. We performed whole YF17D proteome mapping of CTL responses in six Indian rhesus macaques vaccinated with YF17D using overlapping YF17D peptides. We found that the ER luminal E protein was the most immunogenic viral protein followed closely by the cytoplasmic NS3 and NS5 proteins. These results suggest that antigen processing and presentation in this model system is not preferentially affected by the subcellular location of the viral proteins that are the source of CTL epitopes. The data also suggest potential immunogenic regions of YF17D that could serve as the focus of recombinant T cell vaccine development.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag (Germany)
dc.rightsrestricted access
dc.subjectFebre Amarela
dc.subjectVacina
dc.subjectLinfócitos T Citotóxicos
dc.subjectMacaca mulata indiana
dc.subjectYellow Fever 17D
dc.subjectVaccine
dc.subjectCytotoxic T Lymphocyte
dc.subjectIndian Rhesus Macaque
dc.titleThe live-attenuated yellow fever vaccine 17D induces broad and potent T cell responses against several viral proteins in Indian rhesus macaques--implications for recombinant vaccine design
dc.typeArticle


Este ítem pertenece a la siguiente institución