Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil

dc.creatorAugusto, Cláudio José
dc.creatorCarvalho, Wânia da Silva
dc.creatorAlmeida, Isabela Neves de
dc.creatorFigueiredo, Lida Jouca de Assis
dc.creatorDantas, Nayanne Gama Teixeira
dc.creatorSuffys, Philip Noel
dc.creatorMiranda, Silvana Spíndola de
dc.date2019-11-07T17:44:00Z
dc.date2019-11-07T17:44:00Z
dc.date2018
dc.date.accessioned2023-09-26T20:46:17Z
dc.date.available2023-09-26T20:46:17Z
dc.identifierAUGUSTO, Cláudio José et al. Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, v. 49, p. 641-646, 2018.
dc.identifier1517-8382
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36951
dc.identifier10.1016/j.bjm.2017.04.015
dc.identifier1678-4405
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863584
dc.descriptiontDNA genotyping of Mycobacterium tuberculosis has been widely applied in the understandingof disease transmission in many countries. The purpose of this study was to genotype thestrains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais,as well as to compare the similarity, discriminatory power, and agreement of the clustersbetween the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci VariableNumber Tandem Repeat – Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) tech-niques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal toor above 85%. The RFLP-IS6110 and MIRU-VNTR proved to contain a high discriminatorypower. The similarity index resulting from the RFLP showed no recent transmission. Goodagreement was observed between the techniques when clusters were detected; however,the best epidemiological relationship was found when using the RFLP-IS6110.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Microbiologia
dc.rightsopen access
dc.subjectMycobacterium tuberculosis
dc.subjectMinas Gerais
dc.subjectMycobacterium tuberculosis
dc.subjectRFLP
dc.subjectVNTR loci
dc.subjectBrazil
dc.titleComparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
dc.typeArticle


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