Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil

dc.creatorAlmeida, Fernanda
dc.creatorMedeiros, Marta Inês Cazentini
dc.creatorRodrigues, Dália dos Prazeres
dc.creatorFalcão, Juliana Pfrimer
dc.date2016-03-31T16:28:18Z
dc.date2016-03-31T16:28:18Z
dc.date2015
dc.date.accessioned2023-09-26T20:46:10Z
dc.date.available2023-09-26T20:46:10Z
dc.identifierALMEIDA, Fernanda; et al. Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil. Journal of Medical Microbiology, v.64, p.1395-1407, Nov. 2015.
dc.identifier0022-2615
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13423
dc.identifier10.1099/jmm.0.000158
dc.identifier1473-5644
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863552
dc.descriptionSalmonella enterica subsp. enterica serovar Typhimurium is one of the leading serovars that causes salmonellosis worldwide. However, few studies have molecularly characterized S. Typhimurium strains in Brazil. In this study, we genotyped 92 S. Typhimurium strains isolated from humans (43) and food (49) between 1983 and 2013 in Brazil using PFGE, multiple-locus variable number of tandem repeats analysis (MLVA) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Moreover, we assessed the frequency of 12 virulence markers by PCR and the resistance profile against 12 antimicrobials. More than 85.8% of the strains studied carried 11 of the virulence markers or more. Thirty-three strains (25%) were multidrug resistant (MDR). The 92 S. Typhimurium studied were grouped by PFGE as PFGE-A, PFGE-B1 and PFGE-B2; by MLVA as MLVA-A, MLVA-B1 and MLVA-B2; and, finally, by ERIC-PCR as ERIC-A and ERIC-B. The strains isolated from humans before the mid-1990s were allocated to all clusters. The strains isolated from humans after the mid-1990s were distributed in the PFGE-B1, MLVA-B1, MLVA-B2 and ERIC-A clusters. The strains isolated from food were distributed in all clusters, except in PFGE-B2. All typing results suggested that the S. Typhimurium strains of human clinical origin isolated before the mid-1990s were genetically more diverse, which might indicate the selection of a more adapted S. Typhimurium subtype after Salmonella Enteritidis became the most prevalent serovar in Brazil. Regarding strains isolated from food, the results suggest the current circulation of more than one subtype. Furthermore, the high frequency of virulence genes and the presence of MDR strains reinforces their potential hazard for humans and the risk of their presence in foods in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiology Society
dc.rightsrestricted access
dc.subjectMicrobiology
dc.subjectSalmonella Typhimurium
dc.subjectBrazil
dc.subjectGenotypic diversity
dc.subjectDrug resistance
dc.subjectBrasil
dc.subjectSalmonella typhimurium
dc.subjectMicrobiologia
dc.subjectResistência a Medicamentos
dc.titleGenotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil
dc.typeArticle


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