Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting

dc.creatorDopico, Eva
dc.creatorDel-Rei, Rodrigo Pimenta
dc.creatorEspinoza, Bertha
dc.creatorUbillos, Itziar
dc.creatorZanchin, Nilson Ivo Tonin
dc.creatorSulleiro, Elena
dc.creatorMoure, Zaira
dc.creatorCeledon, Paola Alejandra Fiorani
dc.creatorSouza, Wayner Vieira
dc.creatorSilva, Edimilson Domingos da
dc.creatorGomes, Yara Miranda
dc.creatorSantos, Fred Luciano Neves
dc.date2019-04-11T18:23:25Z
dc.date2019-04-11T18:23:25Z
dc.date2019
dc.date.accessioned2023-09-26T20:45:54Z
dc.date.available2023-09-26T20:45:54Z
dc.identifierDOPICO, Eva et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infectious Diseases, v. 19, p. 1-7, 2019.
dc.identifier1471-2334
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32455
dc.identifier10.1186/s12879-019-3872-z
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863469
dc.descriptionThis research project was funded by a Gonçalo Moniz Institute, Oswaldo Cruz Foundation (Fiocruz/BA).
dc.descriptionChronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. Methods: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. Results: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. Conclusions: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in nonendemic settings.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBMC
dc.rightsopen access
dc.subjectDoença de Chagas
dc.subjectTrypanosoma cruzi
dc.subjectAntígenos Quiméricos
dc.subjectImunoensaio
dc.subjectPrecisão
dc.subjectChagas disease
dc.subjectTrypanosoma cruzi
dc.subjectChimeric antigens
dc.subjectImmunoassay
dc.subjectAccuracy
dc.subjectAdulto
dc.subjectAnticorpos Antiprotozoários
dc.subjectImunologia
dc.subjectAntígenos de Protozoários
dc.subjectGenética
dc.subjectDoença de Chagas
dc.subjectDiagnóstico
dc.subjectParasitologia
dc.subjectReações Cruzadas
dc.subjectEmigrantes e Imigrantes
dc.subjectEstatística & dados numéricos
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectFeminino
dc.subjectHumanos
dc.subjectMasculino
dc.subjectGravidez
dc.subjectSensibilidade e Especificidade
dc.subjectEspanha
dc.subjectToxoplasma
dc.subjectTrypanosoma cruzi
dc.titleImmune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
dc.typeArticle


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