Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil

dc.creatorMedeiros, Fernanda Alvarenga Cardoso
dc.creatorSouza Filho, Job Alves de
dc.creatorBarbosa, José Ronaldo
dc.creatorDonato, Lucas Edel
dc.creatorFigueiredo, Fabiano Borges
dc.creatorWerneck, Guilherme Loureiro
dc.creatorPaz, Gustavo Fontes
dc.creatorThompson, Mayerson
dc.creatorMarcelino, Andreza Pain
dc.date2021-06-14T20:42:43Z
dc.date2021-06-14T20:42:43Z
dc.date2021
dc.date.accessioned2023-09-26T20:44:37Z
dc.date.available2023-09-26T20:44:37Z
dc.identifierMEDEIROS, Fernanda Alvarenga Cardoso et al. Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil. Cad. Saúde Pública, v. 37, n. 3, p. 1–11, 2021.
dc.identifier1678-4464
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/47682
dc.identifier10.1590/0102-311X00041320
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863068
dc.descriptionDogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISAVetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.
dc.formatapplication/pdf
dc.languagepor
dc.publisherFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Brasil.
dc.rightsopen access
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectLeishmaniasis, Visceral
dc.subjectDogs
dc.subjectDiagnosis
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.subjectLeishmaniasis Visceral
dc.subjectPerros
dc.subjectTest ELISA
dc.subjectLeishmaniose viscérale
dc.subjectChiens
dc.subjectDiagnostic
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectLeishmaniose Visceral
dc.subjectCães
dc.subjectDiagnóstico
dc.titlePhase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil
dc.typeArticle


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