Diagnostic accuracy of digital RNA quantification versus real-time PCR for the detection of respiratory syncytial virus in nasopharyngeal aspirates from children with acute respiratory infection

dc.creatorBouzas, Maiara Lanna Souza Bacelar
dc.creatorOliveira, Juliana R.
dc.creatorQueiroz, Artur Trancoso Lopo de
dc.creatorFukutani, Kiyoshi Ferreira
dc.creatorBarral, Aldina Maria Prado
dc.creatorRector, Annabel
dc.creatorWollants, Elke
dc.creatorKeyaerts, Els
dc.creatorVan der Gucht, Winke
dc.creatorVan Ranst, Marc
dc.creatorBeuselinck, Kurt
dc.creatorOliveira, Camila Indiani de
dc.creatorVan Weyenbergh, Johan
dc.creatorCarvalho, Cristiana Maria Costa Nascimento
dc.creatorThe Acute Respiratory Infection and Wheeze Study Group Phase I and II
dc.date2018-08-13T13:27:14Z
dc.date2018-08-13T13:27:14Z
dc.date2018
dc.date.accessioned2023-09-26T20:44:28Z
dc.date.available2023-09-26T20:44:28Z
dc.identifierBOUZAS, Maiara Lanna Souza Bacelar et al. Diagnostic accuracy of digital RNA quantification versus real-time PCR for the detection of respiratory syncytial virus in nasopharyngeal aspirates from children with acute respiratory infection. Journal of Clinical Virology, v. 106, p. 34-40, 2018.
dc.identifier1386-6532
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28067
dc.identifier10.1016/j.jcv.2018.07.003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863019
dc.descriptionEthics Committee from the Federal University of Bahia (no 067/2009).
dc.descriptionVirus-specific molecular assays such as real-time polymerase chain reaction (RT-PCR) are regularly used as the gold standard to diagnose viral respiratory tract infections, but simultaneous detection of multiple different pathogens is often challenging. A multiplex digital method of RNA quantification, nCounter (NanoString Technologies), can overcome this disadvantage and identify, in a single reaction, the presence of different respiratory viruses. Objectives: To evaluate the accuracy of nCounter to identify and quantify RSV-A and RSV-B in nasopharyngeal aspirates (NPA) of children (6–23-months-old) with acute respiratory infection. Study design: NPA was collected at enrolment in a prospective cross-sectional study conducted in Salvador, Brazil. A quantitative RT-PCR with a subgroup-specific primer and probeset for RSV-A and RSV-B was performed in parallel with a customized nCounter probeset containing viral targets in NPA. Results: Of 559 NPA tested, RSV was detected by RT-PCR in 139 (24.9%), by nCounter in 122 (21.8%) and by any method in 158 (28.3%) cases. Compared to the gold standard of qRT-PCR, sensitivity of nCounter was 74.3% (95%CI:63.3%–82.9% RSV-A) and 77.6% (95%CI:66.3%–85.9% RSV-B); specificity was 98.4% (95%CI:96.8%–99.2% RSV-A) and 97.8% (95%CI:96.0%–98.8% RSV-B); positive predictive value was 87.3% (95%CI:76.9%–93.4% RSV-A) and 82.5% (95%CI:71.4%–90.0% RSV-B) and negative predictive value was 96.1% (95%CI:94.1%–97.5% RSV-A), and 96.9% (95%CI:95.1%–98.2% RSV-B). Accuracy was 95.2% (95%CI:93.1%–96.7%) for RSV-A and 95.3% (95%CI:93.3%–96.9%) for RSV-B, while both methods significantly correlated for RSV-A (r=0.44, p=8×10−5) and RSV-B (r=0.73, p=3×10-12) quantification. Conclusions: nCounter is highly accurate in detecting RSV-A/B in NPA. Robustness and high-throughput multiplexing indicate its use in large-scale epidemiological studies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectRSV
dc.subjectDiagnóstico
dc.subjectVírus Respiratório
dc.subjectDetecção de Vírus
dc.subjectInfecção respiratória viral
dc.subjectTranscriptômica
dc.subjectRSV
dc.subjectDiagnostics
dc.subjectRespiratory viruses
dc.subjectVirus detection
dc.subjectViral respiratory infection
dc.subjectTranscriptomics
dc.titleDiagnostic accuracy of digital RNA quantification versus real-time PCR for the detection of respiratory syncytial virus in nasopharyngeal aspirates from children with acute respiratory infection
dc.typeArticle


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