Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil

dc.creatorBalassiano, Ilana T.
dc.creatorFilho, Joaquim dos Santos
dc.creatorBrazil, Juliana M. Vital
dc.creatorNouér, Simone A.
dc.creatorSouza, Claudia R. C.
dc.creatorBrazier, Jon S.
dc.creatorFerreira, Eliane de O.
dc.creatorDomingues, Regina M. C. P.
dc.date2018-03-13T15:44:01Z
dc.date2018-03-13T15:44:01Z
dc.date2011
dc.date.accessioned2023-09-26T20:44:28Z
dc.date.available2023-09-26T20:44:28Z
dc.identifierBALASSIANO, Ilana T. et al. Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil. Antonie van Leeuwenhoek, v. 99, 249–255, 2011.
dc.identifier0003-6072
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25280
dc.identifier10.1007/s10482-010-9483-8
dc.identifier1572-9699
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863017
dc.descriptionClostridium difficile is an important nosocomial enteric pathogen and is the etiological agent of pseudomembranous colites. Recently, the rates of C. difficile infection (CDI) have increased worldwide, but in Brazil few data about this situation and the incidence of clonal types of C. difficile exist. This study aimed to isolate and characterize C. difficile strains from samples obtained of a university hospital (HUCFF) in Rio de Janeiro city, Brazil. CDI was identified by ELISA in 27.1% of HUCFF-in-patients enrolled in the study, and the bacterium was recovered from eight of these fecal samples. All strains, except one, presented tcdA and tcdB genes and presented neither the cdtA and cdtB genes nor any significant deletions in the tcdC gene. All strains were sensitive to metronidazole, vancomycin and moxifloxacin, and resistant to clindamycin, ciprofloxacin and levofloxacin. PCR-ribotyping and PFGE revealed four different clonal types among the isolates. The Brazilian PCR-ribotype 133 accounted for 50% of strains isolated, and PCR-ribotype 233 strains were obtained from 25% of the in-patients. The prevalence and resurgence of the Brazilian PCR-ribotype 133 among the hospitalized patients of HUCFF was established, and cross-infection of different patients associated to the same PCR-ribotypes was detected. Our results emphasize the importance of the diagnosis and control of CDI in order to prevent the emergence of specific clones that can lead to C. difficile-associated outbreaks in Brazilian hospitals.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisher1572-9699
dc.rightsrestricted access
dc.subjectClostridium difficile
dc.subjectAntimicrobial resistance
dc.subjectInfecção
dc.subjectRibotipagem
dc.subjectReação em Cadeia da Polimerase
dc.subjectRio de Janeiro
dc.subjectClostridium difficile
dc.subjectClostridium difficile infection
dc.subjectPCR-ribotypes
dc.subjectAntimicrobial resistance
dc.titleDetection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil
dc.typeArticle


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