CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro

dc.creatorRaposo, Jéssica Vasques
dc.creatorBarros, Luciana Rodrigues Carvalho
dc.creatorTorres, Lauana Ribas
dc.creatorPinto, Rafaela Barbosa da Silva
dc.creatorLopes, Amanda de Oliveira
dc.creatorSouza, Elen Mello de
dc.creatorBonamino, Martin Hernan
dc.creatorPaula, Vanessa Salete de
dc.date2023-05-08T19:36:30Z
dc.date2023-05-08T19:36:30Z
dc.date2021
dc.date.accessioned2023-09-26T20:44:04Z
dc.date.available2023-09-26T20:44:04Z
dc.identifierRAPOSO, Jéssica Vasques et al. CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro. Research Square, p. 1 - 10, Oct. 2021.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/58212
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862895
dc.descriptionResumo em português - Google tradutor O HSV-1 afeta aproximadamente 67% da população mundial. Aqui, buscamos utilizar o CRISPR/Cas9 sistema com o alvo UL39, essencial para a replicação do vírus. A sequência de sgRNA foi inserida em plasmídeo (PX459-UL39). As células Vero foram transfectadas com PX459-UL39 e a inibição da replicação viral foi avaliada 24 e 48 horas depois usando ensaios de placa e fluorescência e qPCR. Fluorescência análises revelaram a presença de CRISPR/Cas9 anti-HSV-1 dentro das células Vero, e qPCR mostrou que o a carga viral diminuiu em> 95% das células transfectadas com anti-HSV-1 CRISPR/Cas9. Nossos dados demonstram a utilidade do PX459-UL39 para inibir a infecção por HSV-1.
dc.descriptionHSV-1 affects approximately 67% of the world population. Here, we sought to use the CRISPR / Cas9 system with the UL39 target, essential for virus replication. The sgRNA sequence was inserted into plasmid (PX459-UL39). Vero cells were transfected with PX459-UL39, and inhibition of viral replication was assessed 24 and 48 hours later using plaque assays and fluorescence and qPCR. Fluorescence analyzes revealed the presence of anti-HSV-1 CRISPR/Cas9 within Vero cells, and qPCR showed that the viral load decreased by> 95% of cells transfected with anti-HSV-1 CRISPR / Cas9. Our data demonstrate the usefulness of the PX459-UL39 to inhibit HSV-1 infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherResearch Square
dc.rightsopen access
dc.subjectHSV-1;
dc.subjectsgRNA
dc.subjectFluorescence
dc.subjectCRISPR/Cas9
dc.subjectCRISPR
dc.subjectCas-9 Vector System:
dc.subjectTargets UL-39
dc.subjectInhibits HSV-1 Replication in Vitro
dc.titleCRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
dc.typePreprint


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