Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil

dc.creatorCoutinho, Carlos José P. C. Araújo
dc.creatorBernardino, Thaissa C.
dc.creatorPires, Eder S.
dc.creatorEsch, Lygia V. S.
dc.creatorViviani, Andrea B. P.
dc.creatorCavados, Clara F. G.
dc.date2017-10-19T12:11:29Z
dc.date2017-10-19T12:11:29Z
dc.date2011
dc.date.accessioned2023-09-26T20:43:56Z
dc.date.available2023-09-26T20:43:56Z
dc.identifierCOUTINHO, Carlos José P. C. Araújo; et al. Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil. Journal of Invertebrate Pathology, v.107, p.11–15, 2011.
dc.identifier0022-2011
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/22880
dc.identifier10.1016/j.jip.2011.01.003
dc.identifier1096-0805
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862854
dc.descriptionThe aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in São Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba. Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatment and incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluation of biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresis of crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L(2) fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectCulicidae
dc.subjectLarva
dc.subjectbiodiversidade bacteriana
dc.subjectBacillus thuringiensis
dc.subjectBacillus cereus
dc.subjectBacillus thuringiensis
dc.subjectBacillus cereus
dc.subjectLysinibacillus sphaericus
dc.subjectCulicidae larvae and bacterial biodiversity
dc.titleInvestigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil
dc.typeArticle


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