Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy

dc.creatorGutierrez, Brenda Celeste
dc.creatorAncarola, Maria Eugenia
dc.creatorVolpato-Rossi, Izadora
dc.creatorMarcilla, Antonio
dc.creatorRamirez, Marcel Ivan
dc.creatorRosenzvit, Mara Cecilia
dc.creatorCucher, Marcela
dc.creatorPoncini, Carolina Verónica
dc.date2023-01-12T11:46:37Z
dc.date2023-01-12T11:46:37Z
dc.date2022
dc.date.accessioned2023-09-26T20:43:49Z
dc.date.available2023-09-26T20:43:49Z
dc.identifierGUTIERREZ, Brenda Celeste et al. Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy. Frontiers, v. 12, n. 980817, p. 1–14 , 2022.
dc.identifier2235-2988
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/56442
dc.identifier10.3389/fcimb.2022.980817
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862819
dc.descriptionExtracellular vesicles (EVs) include a heterogeneous group of particles. Microvesicles, apoptotic bodies and exosomes are the most characterized vesicles. They can be distinguished by their size, morphology, origin and molecular composition. To date, increasing studies demonstrate that EVs mediate intercellular communication. EVs reach considerable interest in the scientific community due to their role in diverse processes including antigenpresentation, stimulation of anti-tumoral immune responses, tolerogenic or inflammatory effects. In pathogens, EV shedding is well described in fungi, bacteria, protozoan and helminths parasites. For Trypanosoma cruzi EV liberation and protein composition was previously described. Dendritic cells (DCs), among other cells, are key players promoting the immune response against pathogens and also maintaining self-tolerance. In previous reports we have demonstrate that T. cruzi downregulates DCs immunogenicity in vitro and in vivo. Here we analyze EVs from the in vitro interaction between blood circulating trypomastigotes (Tp) and bone-marrow-derived DCs. We found that Tp incremented the number and the size of EVs in cultures with DCs. EVs displayed some exosome markers and intracellular RNA. Protein analysis demonstrated that the parasite changes the DC protein-EV profile. We observed that EVs from the interaction of Tp-DCs were easily captured by unstimulated-DCs in comparison with EVs from DCs cultured without the parasite, and also modified the activation status of LPS-stimulated DCs.
dc.formatapplication/pdf
dc.languagepor
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectDendritic Cells
dc.subjectCélulas Dendríticas
dc.subjectCellules dendritiques
dc.subjectTrypanosoma cruzi
dc.titleExtracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
dc.typeArticle


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