Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood

dc.creatorRigato, Paula Ordonhez
dc.creatorMaciel, Milton
dc.creatorGoldoni, Adriana Letícia
dc.creatorPiubelli, Orlando
dc.creatorBrito, Cyro Alves de
dc.creatorFusaro, Ana Elisa
dc.creatorAlencar, Liciana Xavier Eurico de
dc.creatorAugust, Thomas
dc.creatorMarques, Ernesto Torres Azevedo
dc.creatorDuarte, Alberto José da Silva
dc.creatorSato, Maria Notomi
dc.date2018-08-31T14:18:27Z
dc.date2018-08-31T14:18:27Z
dc.date2010
dc.date.accessioned2023-09-26T20:42:29Z
dc.date.available2023-09-26T20:42:29Z
dc.identifierRIGATO, P. O. et al. Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood. Virology, v. 406, n. 1, p. 37–47, 10 out. 2010.
dc.identifier1096-0341
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28498
dc.identifier10.1016/j.virol.2010.06.050
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862406
dc.descriptionCNPq ( 141487/2005 ), FAPESP ( 2004 / 14443-2 ), Ministério da Saúde do Brasil / AIDS / DST ( 914BRA1101 ) e LIM-56 / HCFMUSP .
dc.descriptionSuccessful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-gamma, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric LAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectNeonatal
dc.subjectRatos
dc.subjectVacina de DNA
dc.subjectHIV-1
dc.subjectMemória imunológica
dc.subjectNeonatal
dc.subjectMice
dc.subjectDNA vaccine
dc.subjectHIV-1
dc.subjectImmunological memory
dc.subjectVacinas contra a AIDS / genética
dc.subjectVacinas contra a SIDA / imunologia
dc.subjectInfecções por HIV / imunologia
dc.subjectInfecções por HIV / prevenção & controle
dc.subjectHIV -1 / genética
dc.subjectHIV -1 / imunologia
dc.subjectImunização
dc.subjectImunização Secundária
dc.subjectProteína de Membrana Associada aos Lisossomos 1 / genética
dc.subjectProteína 1 de Membrana Associada a Lisossomos / imunologia
dc.subjectPrecursores de Proteína / genética
dc.subjectPrecursores de Proteína / imunologia
dc.subjectVacinas, DNA / administração & dosagem
dc.subjectRatos , endogâmicos BALB C
dc.titleImmunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood
dc.typeArticle


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