Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

dc.creatorSousa, Karina P. de
dc.creatorRossi, Izadora
dc.creatorAbdullahi, Mahamed
dc.creatorRamirez, Marcel Ivan
dc.creatorStratton, Dan
dc.creatorInal, Jameel Malhador
dc.date2023-03-15T19:48:33Z
dc.date2023-03-15T19:48:33Z
dc.date2023
dc.date.accessioned2023-09-26T20:42:07Z
dc.date.available2023-09-26T20:42:07Z
dc.identifierSOUSA, Karina P. de et al. Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. WIREs Nanomed Nanobiotechnol, v. 15, n. e1835, p. 1–29, 2023.
dc.identifier1939-0041
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57396
dc.identifier10.1002/wnan.1835
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862296
dc.descriptionExtracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV-related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens.
dc.formatapplication/pdf
dc.languagepor
dc.publisherWiley
dc.rightsopen access
dc.subjectIsolamento Celular
dc.subjectExtracellular Vesicles
dc.subjectBiomarkers
dc.subjectCell Separation
dc.subjectPathology, Molecular
dc.subjectVesículas Extracelulares
dc.subjectBiomarcadores
dc.subjectSeparación Celular
dc.subjectPatología Molecular
dc.subjectVésicules extracellulaires
dc.subjectMarqueurs biologiques
dc.subjectSéparation cellulaire
dc.subjectAnatomopathologie moléculaire
dc.subjectVesículas Extracelulares
dc.subjectBiomarcadores
dc.subjectSeparação Celular
dc.subjectPatologia Molecular
dc.titleIsolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
dc.typeArticle


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