Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells

dc.creatorBomfim, Diogo S.
dc.creatorFerraz, Rosana Paula Cruz
dc.creatorCarvalho, Nanashara Coelho de
dc.creatorSoares, Milena Botelho Pereira
dc.creatorPinheiro, Maria Lúcia Bélem
dc.creatorCosta, Emmanoel Vilaça
dc.creatorBezerra, Daniel Pereira
dc.date2014-10-09T14:02:22Z
dc.date2014-10-09T14:02:22Z
dc.date2013
dc.date.accessioned2023-09-26T20:42:02Z
dc.date.available2023-09-26T20:42:02Z
dc.identifierBOMFIM, Diogo D. et al. Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells. Basic & Clinical Pharmacology & Toxicology, v. 113, n. 5, p. 300-306, 2013.
dc.identifier1742-7843
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8565
dc.identifier10.1111/bcpt.12097
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862272
dc.descriptionEudesmols are naturally occurring sesquiterpenoid alcohols that present cytotoxic effect to cancer cells. Herein, all eudesmol isomers displayed cytotoxicity to different tumour cell lines. a-Eudesmol showed IC50 values ranging from 5.38 1.10 to 10.60 1.33 lg/mL for B16-F10 and K562 cell lines, b-eudesmol showed IC50 values ranging from 16.51 1.21 to 24.57 2.75 lg/mL for B16-F10 and HepG2 cell lines, and c-eudesmol showed IC50 values ranging from 8.86 1.27 to 15.15 1.06 lg/mL for B16-F10 and K562 cell lines, respectively. In addition, in this work, we studied the mechanisms of cytotoxic action of eudesmol isomers (a-, b- and c-eudesmol) in human hepatocellular carcinoma HepG2 cells. After 24-hr incubation, HepG2 cells treated with eudesmol isomers presented typical hallmarks of apoptosis, as observed by morphological analysis in cells stained with haematoxylin–eosin and acridine orange/ethidium bromide. None of eudesmol isomers caused membrane disruption at any concentration tested. Moreover, eudesmol isomers induced loss of mitochondrial membrane potential and an increase in caspase-3 activation in HepG2 cells, suggesting the induction of caspase-mediated apoptotic cell death. In conclusion, the eudesmol isomers herein investigated are able to reduce cell proliferation and to induce tumour cell death by caspase-mediated apoptosis pathways.
dc.formatapplication/pdf
dc.languageeng
dc.publisherJohn Wiley & Sons Ltd
dc.rightsopen access
dc.subjectEudesmol Isomers
dc.subjectHuman Hepatocellular
dc.subjectCarcinoma HepG2 Cells
dc.subjectApoptose/efeitos de drogas
dc.subjectSesquiterpenos de Eudesmano/farmacologia
dc.subjectCarcinoma Hepatocelular/metabolismo
dc.subjectCarcinoma Hepatocelular/patologia
dc.subjectCaspase 3/genética
dc.subjectCaspase 3/metabolismo
dc.subjectLinhagem Celular Tumoral
dc.subjectMembrana Celular/efeitos de drogas
dc.subjectProliferação de Células/efeitos de drogas
dc.subjectCélulas Hep G2
dc.subjectHumanos
dc.subjectConcentração Inibidora 50
dc.subjectCélulas K562
dc.subjectPotencial da Membrana Mitocondrial/efeitos de drogas
dc.titleEudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
dc.typeArticle


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