Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis

dc.creatorNogueira, Lucas L
dc.creatorCardoso, Fernanda C
dc.creatorMattos, Ana M
dc.creatorBordignon, Juliano
dc.creatorFigueiredo, Cláudia P
dc.creatorDahlstrom, Pedro
dc.creatorFrota, Cristiane C
dc.creatorSantos, Cláudia N. Duarte dos
dc.creatorChalhoub, Marcelo
dc.creatorCavada, Benildo Sousa
dc.creatorTeixeira, Henrique C
dc.creatorOliveira, Sérgio Costa
dc.creatorBarral Netto, Manoel
dc.creatorBafica, Andre Luiz Barbosa
dc.date2014-03-10T14:23:01Z
dc.date2014-03-10T14:23:01Z
dc.date2010
dc.date.accessioned2023-09-26T20:41:33Z
dc.date.available2023-09-26T20:41:33Z
dc.identifierNOGUEIRA, L. et al. Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis. European Journal of Immunology, v. 40, p. 744-753, 2010.
dc.identifier1521-4141
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7386
dc.identifier10.1002/eji.200939747
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862108
dc.descriptionIn this study, we have identified a secreted 13 kDa lectin from Mtb (Mtb, Mycobacterium tuberculosis; sMTL-13) by homology search of a non-redundant lectin database. Bioinformatic analysis revealed that sMTL-13 belongs to the ricin-type beta-trefoil family of proteins containing a Sec-type signal peptide present in Mtb complex species, but not in non-tuberculous mycobacteria. Following heterologous expression of sMTL-13 and generation of an mAb (clone 276.B7/IgG1kappa), we confirmed that this lectin is present in culture filtrate proteins from Mtb H37Rv, but not in non-tuberculous mycobacteria-derived culture filtrate proteins. In addition, sMTL-13 leads to an increased IFN-gamma production by PBMC from active tuberculosis (ATB) patients. Furthermore, sera from ATB patients displayed high titers of IgG Ab against sMTL-13, a response found to be decreased following successful anti-tuberculosis therapy. Together, our findings reveal a secreted 13 kDa ricin-like lectin from Mtb, which is immunologically recognized during ATB and could serve as a biomarker of disease treatment.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.rightsopen access
dc.subjectBiomarkers
dc.subjectLectin
dc.subjectMycobacterium tuberculosis
dc.subjectAntígenos de Bactérias/imunologia
dc.subjectProteínas de Bactérias/imunologia
dc.subjectLectinas/imunologia
dc.subjectMycobacterium tuberculosis/imunologia
dc.subjectTuberculose Pulmonar/imunologia
dc.subjectSequência de Aminoácidos
dc.subjectAnticorpos Antibacterianos/genética
dc.subjectAnticorpos Antibacterianos/imunologia
dc.subjectAntígenos de Bactérias/genética
dc.subjectAntígenos de Bactérias/metabolismo
dc.subjectProteínas de Bactérias/genética
dc.subjectProteínas de Bactérias/metabolismo
dc.subjectSequência de Bases
dc.subjectWestern Blotting
dc.subjectHumanos
dc.subjectImunoglobulina G/imunologia
dc.subjectImuno-Histoquímica
dc.subjectLectinas/genética
dc.subjectLectinas/metabolismo
dc.subjectDados de Sequência Molecular
dc.subjectMycobacterium tuberculosis/genética
dc.subjectMycobacterium tuberculosis/metabolismo
dc.subjectTuberculose Pulmonar/genética
dc.subjectTuberculose Pulmonar/metabolismo
dc.titleMycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis
dc.typeArticle


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