Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)

dc.creatorSouza, Ligia Cristina Kalb
dc.creatorPinho, Rosana Elisa Gonçalves
dc.creatorLima, Carla Vanessa de Paula
dc.creatorFragoso, Stenio Perdigão
dc.creatorSoares, Maurilio José
dc.date2016-09-02T17:22:39Z
dc.date2016-09-02T17:22:39Z
dc.date2013
dc.date.accessioned2023-09-26T20:41:25Z
dc.date.available2023-09-26T20:41:25Z
dc.identifierSOUZA, Ligia Cristina Kalb et al. Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea). Mem. Inst. Oswaldo Cruz, v. 108, n. 5, p. 631-636, 2013..
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15599
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8862071
dc.descriptionCNPq, Fiocruz
dc.descriptionHeteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
dc.formatapplication/pdf
dc.languagepor
dc.rightsopen access
dc.subjectactin
dc.subjectconfocal microscopy
dc.subjecttrypanosomatids
dc.subjectTrypanosoma cruzi
dc.subjectMicroscopia Confocal
dc.subjectTrypanosomatina
dc.subjectActinas
dc.titleActin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
dc.typeArticle


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