Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation

dc.creatorRibeiro Filho, Jaime
dc.creatorLeite, Fagner Carvalho
dc.creatorCalheiros, Andrea Surrage
dc.creatorCarneiro, Alan de Brito
dc.creatorAzeredo, Juliana Alves
dc.creatorAssis, Edson Fernandes de
dc.creatorDias, Celidarque da Silva
dc.creatorPiuvezam, Márcia Regina
dc.creatorBozza, Patrícia T.
dc.date2020-01-21T12:21:53Z
dc.date2020-01-21T12:21:53Z
dc.date2019
dc.date.accessioned2023-09-26T20:41:10Z
dc.date.available2023-09-26T20:41:10Z
dc.identifierRIBEIRO FILHO, Jaime et al. Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation. Toxins, v. 11, n. 12, p. 1-12, 3 Dec. 2019.
dc.identifier2072-6651
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39336
dc.identifier10.3390/toxins11120705
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861989
dc.descriptionPRONEX/MCT
dc.descriptionCNPq
dc.descriptionFAPERJ
dc.descriptionINCT-Câncer
dc.descriptionCurine is a bisbenzylisoquinoline alkaloid (BBA) with anti-allergic, analgesic, and anti-inflammatory properties. Previous studies have demonstrated that this alkaloid is orally active at non-toxic doses. However, the mechanisms underlying its anti-inflammatory effects remain to be elucidated. This work aimed to investigate the effects of curine on macrophage activation and neutrophil recruitment. Using a murine model of lipopolysaccharide (LPS)-induced pleurisy, we demonstrated that curine significantly inhibited the recruitment of neutrophils in association with the inhibition of cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, IL-6, monocyte chemotactic protein (CCL2/MCP-1) as well as leukotriene B4 in the pleural lavage of mice. Curine treatment reduced cytokine levels and the expression of iNOS in in vitro cultures of macrophages stimulated with LPS. Treatment with a calcium channel blocker resulted in comparable inhibition of TNF-α and IL-1β production, as well as iNOS expression by macrophages, suggesting that the anti-inflammatory effects of curine may be related to the inhibition of calcium-dependent mechanisms involved in macrophage activation. In conclusion, curine presented anti-inflammatory effects that are associated with inhibition of macrophage activation and neutrophil recruitment by inhibiting the production of inflammatory cytokines, LTB4 and nitric oxide (NO), and possibly by negatively modulating Ca2+ influx.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectCurine
dc.subjectAlcalóide
dc.subjectMacrófago
dc.subjectNeutrófilo
dc.subjectLipopolissacarídeo
dc.subjectCurine
dc.subjectAlkaloid
dc.subjectMacrophage
dc.subjectNeutrophil
dc.subjectLipopolysaccharide
dc.titleCurine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
dc.typeArticle


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