Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol

dc.creatorBouillet, Leoneide Erica Maduro
dc.creatorDias, Mariana Oliveira
dc.creatorDorigo, Natália Alves
dc.creatorMoura, Andrew Douglas
dc.creatorRussell, Bruce
dc.creatorNosten, Francois
dc.creatorRenia, Laurent
dc.creatorBraga, Erika Martins
dc.creatorGazzinelli, Ricardo Tostes
dc.creatorRodrigues, Mauricio Martins
dc.creatorSoares, Irene da Silva
dc.creatorRomero, Oscar Bruna
dc.date2014-08-04T18:08:15Z
dc.date2014-08-04T18:08:15Z
dc.date2011
dc.date.accessioned2023-09-26T20:41:02Z
dc.date.available2023-09-26T20:41:02Z
dc.identifierBOUILLET, Leoneide Erica Maduro et al. Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol. Infect Immun, 79(9): 3642-3652, 2011.
dc.identifier0019-9567
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8122
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861949
dc.descriptionApical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite’s life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that when it is administered as a recombinant protein (P) in Montanide ISA720 adjuvant, followed by a recombinant human type 5 adenovirus (Ad), intense and long-lasting Plasmodium vivax AMA-1-specific antibody responses (including both IgG1 and IgG2a), as well as proliferative memory T cell responses, can be detected in immunized mice. Memory T cells displayed both central (CD44 hi CD62L hi ) and effector (CD44 hi CD62L lo) phenotypes, with the central memory phenotype prevailing (56% of AMA-1-specific proliferating cells). Considering the main traits of the memory immune responses induced against AMA-1, this particular sequence of immunogens (P followed by Ad), but no others (Ad/Ad, Ad/P, or P/P), displayed an optimal synergistic effect. These results give further support to the need for preclinical studies of P. vivax vaccine candidate AMA-1 administered in prime/boost protocols that include recombinant proteins and adenoviral vectors
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectPlasmodium vivax
dc.subjectvaccines
dc.titleLong-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol
dc.typeArticle


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