Human adipose-derived stem cells reduce cellular damage after experimental spinal cord injury in rats

dc.creatorVialle, Emiliano Neves
dc.creatorFracaro, Letícia
dc.creatorBarchiki, Fabiane
dc.creatorDominguez, Alejandro Correa
dc.creatorArruda, André de Oliveira
dc.creatorOlandoski, Marcia
dc.creatorBrofman, Paulo Roberto Slud
dc.creatorRebelatto, Carmen Lúcia Kuniyoshi
dc.date2023-06-20T16:17:37Z
dc.date2023-06-20T16:17:37Z
dc.date2023
dc.date.accessioned2023-09-26T20:40:41Z
dc.date.available2023-09-26T20:40:41Z
dc.identifierVIALLE, Emiliano Neves et al. Human adipose-derived stem cells reduce cellular damage after experimental spinal cord injury in rats. Biomedicines, v. 11, n. 1394, p. 1-14, 2023.
dc.identifier2227-9059
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/59087
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861831
dc.descriptionTraumatic spinal cord injury (SCI) is a devastating condition without an effective therapy. Cellular therapies are among the promising treatment strategies. Adult stem cells, such as mesenchymal stem cells, are often used clinical research for their immunomodulatory and regenerative potential. This study aimed to evaluate the effect of human adipose tissue-derived stem cells (ADSC) infusion through the cauda equina in rats with SCI. The human ADSC from bariatric surgery was isolated, expanded, and characterized. Wistar rats were subjected to blunt SCI and were divided into four groups. Two experimental groups (EG): EG1 received one ADSC infusion after SCI, and EG2 received two infusions, the first one after SCI and the second infusion seven days after the injury. Control groups (CG1 and CG2) received infusion with a culture medium. In vivo, cell tracking was performed 48 h and seven days after ADSC infusion. The animals were followed up for 40 days after SCI, and immunohistochemical quantification of myelin, neurons, and astrocytes was performed. Cellular tracking showed cell migration towards the injury site. ADSC infusion significantly reduced neuronal loss, although it did not prevent the myelin loss or enhance the area occupied by astrocytes compared to the control group. The results were similar when comparing one or two cell infusions. The injection of ADSC distal to the injured area was shown to be a safe and effective method for cellular administration in spinal cord injury.
dc.formatapplication/pdf
dc.languagepor
dc.publisherMDPI
dc.rightsopen access
dc.subjectSpinal Cord Injuries
dc.subjectCell Therapy
dc.subjectAdipose Tissue
dc.subjectNeurological Rehabilitation
dc.subjectAnimal Experimentation
dc.subjectTraumatismos de la Médula Espinal
dc.subjectTejido Adiposo
dc.subjectRehabilitación Neurológica
dc.subjectExperimentación Animal
dc.subjectTraumatismes de la moelle épinière
dc.subjectThérapie cellulaire et tissulaire
dc.subjectTissu adipeux
dc.subjectRééducation neurologique
dc.subjectExpérimentation animale
dc.subjectTraumas da Medula Espinhal
dc.subjectTerapia Celular
dc.subjectTecido Adiposo
dc.subjectReabilitação Neurológica
dc.subjectExperimentação Animal
dc.titleHuman adipose-derived stem cells reduce cellular damage after experimental spinal cord injury in rats
dc.typeArticle


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