Molecular diversity of disintegrin-like domains within metalloproteinase precursors of Bothrops jararaca

dc.creatorCidade, Daniela A. P.
dc.creatorWermelinger, Luciana S.
dc.creatorLôbo-Hajdu, Gisele
dc.creatorDávila, Alberto M. R.
dc.creatorBon, Cassian
dc.creatorZingali, Russolina B.
dc.creatorAlbano, Rodolpho M.
dc.date2020-01-02T20:01:54Z
dc.date2020-01-02T20:01:54Z
dc.date2006
dc.date.accessioned2023-09-26T20:40:33Z
dc.date.available2023-09-26T20:40:33Z
dc.identifierCIDADE, Daniela A. P. et al. Molecular diversity of desintegrin-like domains within metalloproteinase precursors of Bothrops jararaca. Toxicon, v. 48, n. 5, p. 590-599, Oct. 2006.
dc.identifier0041-0101
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/38952
dc.identifier10.1016/j.toxicon.2006.07.010
dc.identifier1879-3150
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861787
dc.descriptionDisintegrins are small peptides isolated from the venom of several snake families which act as integrin-antagonists or agonists, interacting with a variety of biological processes mediated by integrins. In this work we describe five new disintegrin-like domains within metalloproteinase precursor sequences, obtained from a Bothrops jararaca venom gland cDNA library. Among the new disintegrin-like domains, four were contained in PIII metalloproteinase precursors, with three of them presenting ECD-motifs and one presenting a new KCD-motif. Moreover, we found three disintegrin-like domains within PII metalloproteinase precursors. Two of them are similar to the already described disintegrins jarastatin and jararacin. The third molecule is unusual, presenting some typical PIII metalloproteinase characteristics but lacking the cysteine-rich domain being, thus, classified as a PII metalloproteinase. Only few reports presented molecules with these characteristics. Sequence analysis suggests that these molecules are intermediate steps between the more ancient PIII and the more recent PII metalloproteinases. We also investigated disintegrin N-terminus diversity in B. jararaca crude venom by purifying jarastatin and jararacin and analyzing them by mass spectrometry.
dc.description2025-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectVeneno de serpente
dc.subjectToxina
dc.subjectEvolução
dc.subjectAnálise de purificação e espectrometria de massa
dc.subjectPeptídeos
dc.subjectSnake venom
dc.subjectToxin
dc.subjectDesintegrin
dc.subjectSructure activity relationships
dc.subjectEvolution
dc.subjectPurification and mass spectrometry analysis
dc.titleMolecular diversity of disintegrin-like domains within metalloproteinase precursors of Bothrops jararaca
dc.typeArticle


Este ítem pertenece a la siguiente institución