The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)

dc.creatorSilva, Éverton Fagonde
dc.creatorMedeiros, Marco Alberto
dc.creatorMcBride, Alan John Alexander
dc.creatorMatsunaga, Jim
dc.creatorEsteves, Gabriela S.
dc.creatorRamos, João G. R
dc.creatorSantos, Cleiton Silva
dc.creatorCroda, Julio Henrique Rosa
dc.creatorHomma, Akira
dc.creatorDellagostin, Odir Antonio
dc.creatorHaake, David A.
dc.creatorReis, Mitermayer Galvão dos
dc.creatorKo, Albert Icksang
dc.date2012-11-15T02:21:11Z
dc.date2012-11-15T02:21:11Z
dc.date2007
dc.date.accessioned2023-09-26T20:40:27Z
dc.date.available2023-09-26T20:40:27Z
dc.identifierSILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, n. 33, p. 6277-6286, Aug. 2007.
dc.identifier0264-410X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5818
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861760
dc.descriptionSubunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis.
dc.formatapplication/pdf
dc.languageeng
dc.relationhttps://www.arca.fiocruz.br/handle/icict/23765
dc.rightsopen access
dc.subjectLeptospirosis
dc.subjectSubunit vaccine
dc.subjectLeptospiral immunoglobulin-like protein
dc.subjectRecombinant protein
dc.subjectImmunity
dc.subjectAntibodies
dc.subjectHamsters
dc.subjectProteínas de Bactérias/Imunologia
dc.subjectVacinas Bacterianas
dc.subjectModelos Animais de Doenças
dc.subjectLeptospira/Imunologia
dc.subjectMesocricetus/imunologia
dc.subjectAnimais
dc.titleThe terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
dc.typePreprint


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