Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil

dc.creatorGomes, Fábio Alves
dc.creatorJansen, Ana Maria
dc.creatorMachado, Rosângela Zacarias
dc.creatorPena, Hilda Fátima Jesus
dc.creatorFumagalli, Marcílio Jorge
dc.creatorSilva, Angélica
dc.creatorAlves, Bruna Farias
dc.creatorRoque, André Luiz Rodrigues
dc.creatorFigueiredo, Luiz Tadeu Moraes
dc.date2020-03-30T19:39:06Z
dc.date2020-03-30T19:39:06Z
dc.date2019
dc.date.accessioned2023-09-26T20:39:15Z
dc.date.available2023-09-26T20:39:15Z
dc.identifierGOMES, Fábio Alves et al. Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil. Plos One, v. 14, n. 12, p. 1-13, Dec. 2019.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40563
dc.identifier10.1371/journal.pone.0225895
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861389
dc.descriptionBackground: Arboviruses and protozoans can cause neurologic disorders in horses. In Brazilian Amazon, several horses presenting signs compatible with disorders caused by these infectious agents have been observed. Objective: To contribute to the knowledge of this epidemiological picture, we sought to construct a serological diagnostic panel for neurotrophic infectious agents in local horses. Material and methods: A total of 213 blood samples from horses were collected from 29 farms in three municipalities. Samples were evaluated and considered positive when they met the following criteria: titers ≥ 1:80 with the indirect fluorescent antibody test (IFAT) for apicomplexan protozoans; positive recombinant enzyme-linked immunosorbent assay (ELISA) with subsequent titers ≥ 1:10 by the PRNt for viruses; and detection under direct microscopic examination for Trypanosoma evansi. Results: No horses were found to be infected by T. evansi, and only two were infected Toxoplasma gondii and/or Neospora spp. The highest protozoan infection rate was observed for Sarcocystis neurona (40.3%; n = 86/213). Among the positive ELISA samples tested by the plaque reduction neutralization test (PRNT90), 92% (n = 76/83) were positive for St Louis Encephalitis virus, 43% (n = 6/14) were positive for West Nile virus and 33% (n = 16/48) were positive for Mayaro virus. Eighteen percent (n = 39/213) of horses were co-infected by S. neurona and at least one arbovirus, particularly SLEV and/or MAYV. Conclusion: Samples positive for SLEV associated with S. neurona, including samples from horses that had recovered from neurological signs were frequent, and must be considered when investigating the possible causes of neurological diseases in South Roraima horses.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectEvidência sorológica
dc.subjectCoccídios
dc.subjectArbovírus
dc.subjectInfecção
dc.subjectCavalos
dc.subjectAmazônia Brasileira
dc.subjectSerological evidence
dc.subjectArboviruses
dc.subjectCoccidia
dc.subjectInfection
dc.subjectHorses
dc.subjectBrazilian Amazon
dc.titleSerological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil
dc.typeArticle


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