Is there a relationship between the detection of human herpesvirus 8 and Epstein-Barr virus in Waldeyer's ring tissues?

dc.creatorChagas, Cristiano Aparecido
dc.creatorEndo, Luiza Hayashi
dc.creatordosSantos, Washington Luis Conrado
dc.creatorPinto, Glauce Aparecida
dc.creatorSakano, Eulália
dc.creatorBrousset, Pierre
dc.creatorVassallo, José
dc.date2014-05-28T16:28:46Z
dc.date2014-05-28T16:28:46Z
dc.date2006
dc.date.accessioned2023-09-26T20:39:12Z
dc.date.available2023-09-26T20:39:12Z
dc.identifierCHAGAS, C. A. et al. Is there a relationship between the detection of human herpesvirus 8 and Epstein-Barr virus in Waldeyer's ring tissues? International Journal of Pediatric Otorhinolaryngology, v. 70, p. 1923—1927, 2006.
dc.identifier0165-5876
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7660
dc.identifier10.1016/j.ijporl.2006.06.020
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861377
dc.descriptionOBJECTIVE: Human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV) are human pathogens associated to a number of neoplasms, including tumors of the Waldeyer's ring. Both viruses have been previously detected by in situ methods in tonsils and adenoids from children. HHV-8 was found in 6.8% of the cases and EBV in about one third of the cases. As they belong to the same gamma-herpesvirus subfamily and share some biological characteristics, it is of medical interest to further explore their possible relationship in the Waldeyer's ring, an issue not yet addressed in the specialized literature. The purpose of the present study is to compare the presence of EBV by in situ hybridization (ISH) in tonsils and adenoids from children up to 14 years of age in cases previously shown to be positive and negative for HHV-8. METHODS: Paraffin wax-embedded sections consisting of 38 tonsils and two adenoids from 40 patients were analyzed. HHV-8 was detected by ISH, using the T1-1 probe for the viral mRNA. EBV was also detected by ISH, using the EBER probe. Both probes and the detection systems were provided by Novocastra. RESULTS: HHV-8 was detected in 19 tonsils and one adenoid. The other 19 tonsils and one adenoid taken from the HHV-8-negative group were selected by pairing age and gender of patients with the HHV-8-positive group. In both groups EBV was detected in 13 cases and was negative in other 7. CONCLUSION: Although both viruses are related in many aspects, some biological and epidemiological features differ. This is reflected in the present results, as EBV is similarly detected in the groups negative and positive for HHV-8, favoring different mechanisms of spread.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectHuman herpesvirus 8
dc.subjectEpstein—Barr virus
dc.subjectTonsils
dc.subjectAdenoids
dc.subjectIn situ hybridization
dc.subjectTonsila Faríngea/virologia
dc.subjectHerpesvirus Humano 4/isolamento & purificação
dc.subjectTonsila Palatina/virologia
dc.subjectTonsila Faríngea/química
dc.subjectAdolescente
dc.subjectCriança
dc.subjectPré-Escolar
dc.subjectDNA Viral/análise
dc.subjectFeminino
dc.subjectHumanos
dc.subjectHibridização In Situ
dc.subjectLactente
dc.subjectMasculino
dc.subjectTonsila Palatina/química
dc.subjectInclusão em Parafina
dc.titleIs there a relationship between the detection of human herpesvirus 8 and Epstein-Barr virus in Waldeyer's ring tissues?
dc.typeArticle


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