Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: evaluation by two molecular markers

dc.creatorBaptista, C.
dc.creatorSchubach, A. O.
dc.creatorMadeira, M. F.
dc.creatorLeal, C. A.
dc.creatorPires, M. Q.
dc.creatorOliveira, F. S.
dc.creatorSilva, Fátima Conceição
dc.creatorRosalino, C. M. V.
dc.creatorSalgueiro, M. M.
dc.creatorPacheco, R. S.
dc.date2018-08-16T11:53:38Z
dc.date2018-08-16T11:53:38Z
dc.date2009
dc.date.accessioned2023-09-26T20:39:03Z
dc.date.available2023-09-26T20:39:03Z
dc.identifierBAPTISTA, C. et al. Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: Evaluation by two molecular markers. Experimental Parasitology, v. 121, p. 317-322, 2009.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28167
dc.identifier10.1016/j.exppara.2008.12.006
dc.identifier1090-2449
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861333
dc.descriptionAnalyses of MLEE, RAPD and LSSP-PCR were used to compare the panel of american tegumentary leishmaniasis (ATL) isolates obtained from lesions of patients with rare clinical manifestations of the disease and typical lesions. All of the 34 samples analyzed by MLEE demonstrated similar electromorphic profiles with Leishmania (Viannia) braziliensis reference strain. Through the RAPD analysis, nine genetic profiles (genotypes) were identified. LSSP-PCR corroborates the initial screening and phenetic analysis has grouped the isolates into two major clusters comprising the nine different genotypes. Prevalent genotype defined as LbmtDNAgen1 was detected in the largest number of isolates. There was no association between genotypes and clinical symptoms. However, two different genotypes could be identified in the initial (LbmtDNAGen9) and reactivated lesion (LbmtDNAGen3) of the same patient. Our results support the idea of a less pronounced genotypic diversity among L. (V.) braziliensis circulating in the State of Rio de Janeiro and demonstrate the useful application of these molecular markers in genetics variability studies.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectLeishmaniose
dc.subjectEletroforese enzimática multilocus
dc.subjectReação em Cadeia da Polimerase
dc.subjectDNA polimórfico amplificado randomizado
dc.subjectDNA de Cinetoplasto
dc.subjectVariabilidade genética
dc.subjectLeishmania (Viannia) braziliensis
dc.subjectLeishmaniasis
dc.subjectMultilocus enzyme electrophoresis
dc.subjectRandom amplified polymorphic DNA
dc.subjectLow stringency single specific
dc.subjectPrimer-polymerase chain reaction
dc.subjectKinetoplast DNA
dc.subjectGenetic variability
dc.titleLeishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: evaluation by two molecular markers
dc.typeArticle


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