Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy

dc.creatorBarros, L. A.
dc.creatorNeves, R. H.
dc.creatorMoura, E. G. de
dc.creatorSilva, J. R. Machado
dc.date2019-01-10T12:18:33Z
dc.date2019-01-10T12:18:33Z
dc.date2009
dc.date.accessioned2023-09-26T20:38:16Z
dc.date.available2023-09-26T20:38:16Z
dc.identifierBARROS, L. A. et al. Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy. Journal of Helminthology, v. 83, p.13-21, 2009.
dc.identifier0022-149X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30953
dc.identifier10.1017/S0022149X08067217
dc.identifier1475-269
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861077
dc.descriptionPrevious studies have shown that protein deficiencies can hamper both the course of experimental schistosomiasis and normal development of adult worms. To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphometric and confocal laser scanning microscopy analysis. Swiss mice were fed protein-deficient diets (8%) and infected subcutaneously with approximately 80 S. mansoni cercariae (BH strain, Brazil). Control mice were fed a standard rodent diet (23% protein). The nutritional status was evaluated by body weight gain and albumin values. Mice were sacrificed 63 days post-infection. Recovered worms were stained with hydrochloric carmine and preserved as whole-mounts for bright-field examination and confocal microscopy. The body weight gain and serum albumin concentrations were significantly lower (P < 0.05) in malnourished mice than in controls. In general, all morphometric values of specimens grown in malnourished mice were lower than those of control mice. Schistosome worms grown in malnourished mice had statistically significant differences (P < 0.05) in the reproductive system and tegument than those grown in mice fed standard diets. In female worms, vitelline glands showed few remaining follicles and ovaries lacked mature oocytes. In male parasites, tubercles were fewer in number on the dorsal surface and testicular lobes presented fewer differentiated germinal cells. In summary, we describe novel data supporting the view that low-protein diets may influence the development of adult worms.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectSchistosoma mansoni
dc.subjectMorfologia
dc.subjectMicroscopia confocal de varredura a laser
dc.subjectDieta baixa em proteínas
dc.subjectSchistosoma mansoni
dc.subjectMorphology
dc.subjectConfocal laser scanning microscopy
dc.subjectLow-protein diet
dc.titleEffects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy
dc.typeArticle


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