Unveiling alterative splice diversity from human oligodendrocyte proteome data

dc.creatorTavares, Raphael
dc.creatorWajnberg, Gabriel
dc.creatorScherer, Nicole de Miranda
dc.creatorPauletti, Bianca Alves
dc.creatorCassoli, Juliana S.
dc.creatorFerreira, Carlos Gil
dc.creatorLeme, Adriana Franco Paes
dc.creatorSouza, Patricia Savio de Araujo
dc.creatorSouza, Daniel Martins de
dc.creatorPassetti, Fabio
dc.date2018-03-08T12:16:43Z
dc.date2018-03-08T12:16:43Z
dc.date2017
dc.date.accessioned2023-09-26T20:38:03Z
dc.date.available2023-09-26T20:38:03Z
dc.identifierTAVARES, Raphael; et al. Unveiling alterative splice diversity from human oligodendrocyte proteome data. Journal of Proteomics, v.151, p.293-301, May 2017.
dc.identifier1874-3919
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25182
dc.identifier10.1016/j.jprot.2016.05.023
dc.identifier1876-7737
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860999
dc.descriptionOligodendrocytes produce and maintain the myelin sheath of axons in the central nervous system. Because misassembled myelin sheaths have been associated with brain disorders such as multiple sclerosis and schizophrenia, recent advances have been made towards the description of the oligodendrocyte proteome. The identification of splice variants represented in the proteome is as important as determining the level of oligodendrocyte-associated proteins. Here, we used an oligodendrocyte proteome dataset deposited in ProteomeXchange to search against a customized protein sequence file containing computationally predicted splice variants. Our approach resulted in the identification of 39 splice variants, including one variant from the GTPase KRAS gene and another from the human glutaminase gene family. We also detected the mRNA expression of five selected splice variants and demonstrated that a fraction of these have their canonical proteins participating in direct protein-protein interactions. In conclusion, we believe our findings contribute to the molecular characterization of oligodendrocytes and may encourage other research groups working with central nervous system disorders to investigate the biological significance of these splice variants. The splice variants identified in this study may encode proteins that could be targeted in novel treatment strategies and diagnostic methods.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectBioinformática
dc.subjectProteogenômica
dc.subjectProteômica
dc.subjectOligodendroglia
dc.subjectProcessamento Alternativo
dc.subjectAlternative splicing
dc.subjectBioinformatics
dc.subjectOligodendrocytes
dc.subjectProteogenomics
dc.subjectProteomics
dc.titleUnveiling alterative splice diversity from human oligodendrocyte proteome data
dc.typeArticle


Este ítem pertenece a la siguiente institución